Abstract：Comprehensive Laboratory of Biochemistry and Molecular Biology is a compulsory open online course that integrates lab skill training and innovation ability training for undergraduates majoring in biology in Beijing Normal University. This course focuses on alkaline phosphatase, including cloning of the Escherichia coli alkaline phosphatase gene, expression and purification of the protein, research on its enzymatic characteristics, preparation of its antibody and determination the titers of the antibody, etc. The comprehensive lab course covers from the gene to the protein, so that students can learn and master commonly used biochemical and molecular biology experimental techniques systematically. Escherichia coli alkaline phosphatase is an ideal experimental research object. And there are very classic and systematic scientific research literature reports. As important auxiliary teaching means, these literature resources can help teachers and students to carry out learning inquiries, form rational and critical scientific attitudes and spirits, and also greatly improve the quality of teaching and students′ scientific research quality, which plays an important role in the construction of first-class course of Comprehensive Laboratory of Biochemistry and Molecular Biology.
尹燕霞, 杨冬, 佟丽, 李森, 向本琼, 骆静. 经典文献阅读在《生物化学与分子生物学综合实验》课程建设中的应用[J]. 中国生物化学与分子生物学报, 2021, 37(10): 1408-1414.
YIN Yan-Xia, YANG Dong, TONG Li, LI Sen, XIANG Ben-Qiong, LUO Jing. Application of Classical Literature Reading in the Course of Comprehensive Laboratory of Biochemistry and Molecular Biology. Chinese Journal of Biochemistry and Molecular Biol, 2021, 37(10): 1408-1414.
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