Abstract：Shugoshin-1 (SGOL1) is one of the human homologues of yeast Shugoshin that locates in the centromeric region. It prevents premature division of the centromeric cohesin complex and maintaining chromosome stability. Very recently, the role of SGOL1 in tumors has emerged, but its role in lung cancer is unclear. In this study, we identify that SGOL1 was upregulated in lung cancer samples from the TCGA database (n=529, P<0.00001), which was correlated to the overall survival time of lung cancer patients (P=0.0049). Detected by qRT-PCR and Western blotting, we demonstrate that SGOL1 was consistently upregulated in lung cancer cell lines than normal epithelial cell line. Regulatory effects of SGOL1 on cell viability, clonality, migration and invasion of lung cancer cells A549 and NCI-H2405 were examined by Cell Counting Kit-8 (CCK8), Colony formation, Scratch and Transwell assays, respectively. Compared with the control group, knockdown of SGOL1 significantly inhibited proliferation, migration and invasion in A549 and NCI-H2405 cells (P<0.05). A positive correlation was identified between expression levels of pyruvate kinase muscle isoenzyme 2 (PKM2) and SGOL1 in the TCGA database (r=0.38, P=0). Western blotting results showed that knockdown of SGOL1 in A549 and NCI-H2405 cells down-regulated PKM2 as well. In addition, co-interventions of SGOL1 and PKM2 were performed in A549 and NCI-H2405 cells to clarify the interaction mechanism between them. The results showed that overexpression of PKM2 in lung cancer cells could partially reverse the regulatory effects of SGOL1 knockdown on proliferation, migration and invasion (P<0.05). Therefore, our study showed that SGOL1 promoted the proliferation, migration and invasion of lung cancer cells by regulating PKM2. This study provided a theoretical basis for the mechanism of SGOL1 in lung cancer.
邓博云, 傅文凡, 江泽勇, 戴路, 钟圣鹏, 赵健. SGOL1通过调节PKM2促进肺癌细胞增殖和侵袭[J]. 中国生物化学与分子生物学报, 2021, 37(10): 1394-1400.
DENG Bo-Yun, FU Wen-Fan, JIANG Ze-Yong, DAI Lu, ZHONG Sheng-Peng, ZHAO Jian. SGOL1 Promotes Proliferation and Invasion of Lung Cancer Cells Through PKM2. Chinese Journal of Biochemistry and Molecular Biol, 2021, 37(10): 1394-1400.
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