Abstract：Although chondrocyte energy metabolism is dominantly glycolytic rather than through oxidative phosphorylation, mitochondrial dysfunction has been reported to be involved in the process of cartilage degeneration. Mitochondria in articular chondrocytes did not get much attention because of avascular and physiologically hypoxic environment in cartilage. Previous studies were limited to observe the mitochondrial morphology and superoxide anion of primary chondrocytes isolated and cultured in vitro, which were significantly different from in-situ chondrocytes in terms of morphological and metabolic characteristics. We established a set of novel in situ optical imaging approaches, and found that mitochondrial abundance as well as chondrocyte density declines with distance from the surface of intact cartilage. Other study has shown that in chondrocytes, mitochondrial derived reactive oxygen species (ROS) can work with pro-inflammatory cytokines to accelerate cartilage degeneration. Superoxide anion produced by electron leakage in mitochondrial respiratory chain is the most important source of intracellular ROS, and superoxide anion has a very short half-life and is easily converted to hydrogen peroxide spontaneously, making it the most difficult ROS signal to detect. This study was undertaken to make a morphometric analysis of mitochondria in articular chondrocytes using confocal micrographs and fluorescent probes, and to explore possible role of mitochondrial oxidative stress in inflammatory response in articular chondrocytes. The results suggested that mitochondria were probably one of the essential organelles for articular chondrocytes. The superoxide flash response to proinflammatory cytokine stimulation suggests that chondrocyte mitochondria are a significant source of cellular oxidant and an important mediator which was previously under-appreciated in inflammatory cartilage diseases.
胡晓青，程 锦， 张 辛， 敖英芳. 基于超氧阴离子探针观察原位软骨细胞线粒体及线粒体炫
[J]. 中国生物化学与分子生物学报, 2020, 36(7): 858-864.
HU Xiao-Qing, CHENG Jin, ZHANG Xin, AO Ying-Fang. Real-time Monitoring of Mitochondria and Mitochondrial Flashes of Chondrocytes in Situ Based on Superoxide Anion Probe. Chinese Journal of Biochemistry and Molecular Biol, 2020, 36(7): 858-864.
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