Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a house-keeping glycolysis gene highly and constantly expressed in almost every tissue. So it is widely used as a relative reference to quantitate proteins, RNA and DNA. But in recent years, GAPDH has been questioned as an internal reference, especially in tumor and aging tissues. Extensive studies have indicated that GAPDH is up-regulated in tumors, and down-regulated in aging muscles. The high expression levels of GAPDH in tumors may be associated with tumor metastasis and proliferation. In this review, we cover the expression of GAPDH in tumor and aging tissues or cells, as well as the possible underlying mechanisms. We aim to provide a blueprint of GAPDH expression in these pathological and physiological processes, facilitating investigators to select a better internal reference in future molecular experiments.
郑琳, 郑慧玲, 刘新光. 管家基因GAPDH在肿瘤与衰老组织中作为内参应慎重[J]. 中国生物化学与分子生物学报, 2018, 34(4): 385-389.
ZHENG Lin, ZHENG Hui-Ling, LIU Xin-Guang. GAPDH Should Be Cautiously Used as an Internal Reference in Tumor and Aging Tissues. Chinese Journal of Biochemistry and Molecular Biol, 2018, 34(4): 385-389.
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