(1) Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;
2)College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
3)National Drug Screening Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China)
To investigate whether wogonoside has an anti-aging role, we studied its effect on the natural lifespan of Drosophila melanogaster. Metabonomics techniques, such as RT-PCR and UPLC-MS/MS, were used to explore the potential anti-aging effect of wogonoside. The results showed that 0.02 mg/mL and 0.5 mg/mL wogonoside could significantly prolong the overall longevity of fruit flies and improve the mean lifespan by 5.64% and 5.39%, and the maximum lifespan by 2.74% and 5.12%, respectively. Dietary supplement of wogonoside significantly up-regulated the expression of antioxidant genes SOD1, SOD2 and CAT, and down-regulated the expression of MTH in fruit flies, compared with the 30 d group. Seventeen potential biomarkers regulated by wogonoside treatment were found. The metabolic pathway analysis suggested that wogonoside antagonizes senescence mainly through modulating amino acid metabolism (D-glutamine and D-glutamate metabolism; alanine, aspartate and glutamate metabolism; arginine and proline metabolism; valine, leucine and isoleucine biosynthesis) and energy metabolism (nitrogen metabolism). The results suggest that the anti-aging role of wogonoside is associated with increasing the expression of antioxidant genes and regulating different metabolic pathways.
薛立英,高丽, 秦雪梅, 杜冠华, 周玉枝. 汉黄芩苷通过调节抗氧化基因表达和机体代谢延长果蝇寿命[J]. 中国生物化学与分子生物学报, 2018, 34(4): 404-416.
XUE Li-Ying, GAO Li QIN Xue-Mei, DU Guan-Hua, ZHOU Yu-Zhi. Wogonoside Extends the Lifespan of Drosophila melanogaster by Regulating the Expression of Antioxidant Genes and Body Metabolism. Chinese Journal of Biochemistry and Molecular Biol, 2018, 34(4): 404-416.
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