Angiogenin (ANG) regulates cell proliferation, migration, differentiation and other biological processes, but the molecular mechanism of ANG function is not fully clear. It has been reported that ANG bound rDNA to enhance rRNA transcription, or might also bind to mRNA coding regions. To screen and identify angiogenin binding sites genome-wide, we employed the technology of chromatin immunoprecipitation coupled with microarray (ChIP-chip) and identified a total of 1 248 binding sites using genomic DNA from HeLa cells. We found 699 potential angiogenin-regulating genes by annotating the genes near the binding sites. Gene annotation and cluster analysis results showed that these genes associated to tumorigenesis and progression linked to TGF-β and Wnt signaling pathway, especially for colorectal and prostate cancers. We verified that angiogenin bound to the promoter regions of the WNT6, CCNE1, APC2, FZD8 and EGFR genes to regulate their expression. These findings provided clues for understanding the mechanisms of angiogenin functions.
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