Screening 50 Hz Pulsed Electromagnetic Fields of Different Intensities on Proliferation, Differentiation and Mineralization of Rat Osteoblast for the Optimal Parameters
YAN Juan-Li1）， WANG Ming-Gang1）*， CHENG Ke-Ming2）， GE Bao-Feng2）， ZHOU Jian2）， MA Xiao-Ni2）， SONG Peng2）， CHENG Kui 2）
(1)School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou730050, China;2)Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou730050, China)
Abstract：To study the effects of pulsed electromagnetic fields (PEMFs) of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblast (OB), osteoblast cells were sub-cultured and randomly divided into seven groups: 0, 0.6, 1.2, 1.8, 2.4, 3.0, and 3.6 mT, respectively (with a 0.6 mT increment interval). MTT result showed that treatment at 0.6 mT, 1.8 mT and 3.6 mT induced a signifcant increase of osteoblast proliferation. Treatment at 0.6 mT signifcantly increased osteoblast proliferation (P<0.01). Treatments at 50Hz 0.6 mT signifcantly increased alkaline phosphatase (ALP) activity and the amount of calcium deposition (P<0.01) than that in control groups. The osteoblasts increased the formation of calcified tubercle from 4 days to 12 days after PEMFs exposure. Treatments at 50 Hz 0.6 mT significantly increased the amount of calcium deposition (P<0.01). Consistently, treatment at 0.6 mT signifcantly increased the number and area of histochemically stained (P<0.01), as well as alizarin red-stained ALP compared to the untreated control. mRNA expression showed that exposure at 0.6mT signifcantly increased Bmp-2 and Collagen-1 mRNA expression compared to the untreated control (P<0.01). Exposure to PEMFs of different intensities signifcantly decreased Rankl mRNA expression compared to the untreated control. Exposure at 50 Hz 0.6 mT PEMFs significantly enhanced osteoblast proliferation, differentiation and mineralization. It is a theoretical basis that pulsed electromagnetic fields were used to treat osteoporosis.
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YAN Juan-Li WANG Ming-Gang CHENG Ke-Ming GE Bao-Feng ZHOU Jian MA Xiao-Ni SONG Peng CHENG Kui . Screening 50 Hz Pulsed Electromagnetic Fields of Different Intensities on Proliferation, Differentiation and Mineralization of Rat Osteoblast for the Optimal Parameters. Chinese Journal of Biochemistry and Molecular Biol, 2014, 30(7): 721-729.
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