Abstract：Enzymatic synthesis of desheptapeptide insulin amide was carried out in aqueous solution in the presence of sucrose.The coupling yield was found to be 45.5%.This medium is an alternative to the co-solvent system commonly used in enzymatic peptide synthesis and may find applications in dealing with hydrophilic peptides and unstable enzymes.
崔大敷,范利,张友尚. 在蔗糖水溶液中去七肽(B24-B30)胰岛素酰胺(DHpI·NH_2)的酶促合成[J]. 中国生物化学与分子生物学报, 1986, 2(02): 33-36.
Cui,Da-fu Fan,Li Zhang,You-Shang. ENZYMATIC SYNTHESIS OF DESHEPTAPEPTIDE(B24-B30) INSULIN AMIDE IN AQUEOUSSOLUTION OF SUCROSE. Chinese Journal of Biochemistry and Molecular Biol, 1986, 2(02): 33-36.
