Effect of LRP16 on Breast Cancer MCF-7 Cell Proliferation
HAN Wei dong 1) , LI Xue ling 2) , LU Xue chun 3) , XU Zhou min 3) , YU Li 3) , LI Ming 4) , MU Yi ming 4)*
( 1) Department of Molecular Biology, Institute of Basic Medicine, Chinese PLA General Hospital,Beijing 100853; 2) Central Laboratory, Dalian Central Hospital, Dalian 116033; 3) Department of Hematology, Chinese PLA General Hospital,Beijing 100853; 4) Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China
Abstract：The expression level of LRP16 mRNA induced by 17β estrodiol (17β E 2) in MCF 7 cells was determined by Northern blot analysis. LRP16 promoter controlled luciferase expression vector (pS 0) was constructed and was co transfected with estrogen receptor α and β (ERα and ERβ) into COS 7 and MCF 7 cells, respectively, then the relative luciferase activity was measured. The effect of overexpression of LRP16 on MCF 7 cell proliferation was examined by trypan blue exclusion method in the cells transfected by LRP16. The results indicated that 17β E 2 increased the expression of LRP16 mRNA in MCF 7 cells, and this effect was not time and dose dependent. The relative luciferase activity in both COS 7 cells and MCF 7 cells co transfected by pGL3 S 0 and ERα was 5 to 10 fold of that in the control cells transfected by pS 0 alone or co transfected by pS 0 and ERβ. Overexpression of LRP16 promoted MCF 7 cell proliferation. These results suggested that estrogen up regulated the expression level of LRP16 mRNA through activating ERα and promoted MCF 7 cell proliferation.
韩为东,李雪岭,卢学春,徐周敏,于力,李明,母义明. LRP16对乳腺癌MCF-7细胞增殖的影响[J]. 中国生物化学与分子生物学报, 2003, 19(05): 672-676.
HAN Wei dong 1) , LI Xue ling 2) , LU Xue chun 3) , XU Zhou min 3) , YU Li 3) , LI Ming 4) , MU Yi ming 4)*. Effect of LRP16 on Breast Cancer MCF-7 Cell Proliferation. Chinese Journal of Biochemistry and Molecular Biol, 2003, 19(05): 672-676.
