Abstract：Representational difference analysis of cDNA(cDNA RDA) was applied to identify differences of gene expression in renal tissue of the type 2 diabetic rats and study the underlying molecular machanism of diabetes and its related complications.The difference bands were generated by cDNA RDA. The final difference products was subcloned into the pUC 18 vector and sequenced. The expressed sequence tags (ESTs) were analysed by bioformatics and the expression levels of ESTs and genes were then verified by semi quantitative RT PCR primitively. 9 novel ESTs and 2 novel genes were obtained in renal tissue of the rat. These novel genes were closely similar to serine (or cysteine) proteinase inhibitor, clade F and eukaryotic translation initiation factor 3, subunit 5 (EIF 3 epsilon) and up regulated in renal tissue of the type 2 diabetic rats. Full length cDNA of a novel gene (RS91) was cloned by in silico cloning. It is presumed that the novel genes may be serine proteinase inhebitor, clade F, and EIF 3 epsilon of rats, respectively and may be related to renal impairment of the type 2 diabetic rat.
杨架林,李果,张芳林,刘优萍,张迪,周文中,许光武,杨义生,罗敏. cDNA RDA在类似普通2型糖尿病大鼠肾脏组织基因差异表达筛查中的应用[J]. 中国生物化学与分子生物学报, 2003, 19(05): 667-671.
YANG Jia lin, LI Guo, ZHANG Fang lin, LIU You ping, ZHANG Di, ZHOU Wen zhong, XUE Guang wu, YANG Yi sheng, LUO Min *. Identifying Differences of Gene Expression in Renal Tissue of Type 2 Diabetic Rats by Representational Difference Analysis of cDNA. Chinese Journal of Biochemistry and Molecular Biol, 2003, 19(05): 667-671.
