Abstract：In order to study whether there is a functional regulation region within the 3′untranslational region (UTR) of human phosphoinositide 3 kinaseγ(PI3Kγ)mRNA, the 3′UTR was analyzed through bioinformatics. An AU rich region (AUR)of about 0 9 kb was found. Within the AU rich region there are 4 AU rich elements (AREs) and a region which is homologous with many other UTRs. The AU rich region was inserted into the 3′UTR of egfp reporter gene, and pcDNA3 egfp AUR expressing vector was constructed. The expression of egfp containing the 3′UTR of PI3Kγ was significantly reduced by 2～3 times in NIH 3T3, 7402 and K562 cells. After treated with actinomycin D (5 μg/ml) total RNA from stably transfected K562 cells were successively extracted with an interval of 2 hours, and the stability of egfp mRNA was analyzed through Northern blotting. The result showed that the AU rich region of PI3Kγ could, to some extent, accelerate the decay of egfp mRNA. These results indicate that there is probably a negative regulation region within the 3′AU rich region of PI3Kγ mRNA 3′UTR.
戴冰冰,卢健,王家敏,梅文瀚,王楚,钱关祥. PI3KγmRNA 3′非翻译区可能存在基因表达负调控区[J]. 中国生物化学与分子生物学报, 2003, 19(05): 630-635.
DAI Bing bing, LU Jian, WANG Jia min, MEI Wen han, WANG Chu, QIAN Guan xiang *. Probable Existence of a Negative Regulation Region Within the 3′UTR of Human Phosphoinositide 3-Kinaseγ mRNA. Chinese Journal of Biochemistry and Molecular Biol, 2003, 19(05): 630-635.
