Transcription of A Mitochondrial GIY-YIG Family Homing Endonucleases Gene Enhanced in Dunaliella salina Under Salt Stress
FANG Xiao dong 1), 2) , LIN Qi feng 2) , LI Guan yi 2) , QU Liang hu 1)
( 1) The Key Laboratory of Gene Engineering of the Ministry of National Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China; 2) Hainan Provincial Key Laboratory of Biotechnology for Salt tolerance Crops, Institute of Biological Science and Technology, Hainan University, Haikou 570228, China
Abstract：Outstanding salt tolerance ability has made Dunaliella salina (Chlorophyta) an important model organism for clarifying the molecular mechanisms of salt tolerance. An isolated Dunaliella salina gene fragment up expressed under salt stress was studied further. 5′ end cDNA sequence and part 3′ end cDNA sequence were obtained through RACE reaction. The sequence analysis showed that this sequence represented a mitochondrial group I intronic gene coding for GIY YIG family homing endonucleases. Combination of RNase free DNase digestion with Northern blot analysis showed that mitochondrial DNA of Dunaliella salina might be amplified under salt stress. These results suggested that the up expression of this intronic gene might be a by product of the process of salt tolerance, which was unnecessary for the salt tolerance of Dunaliella salina , and that the gene containing this intronic gene might be the genuine gene that needed to be up expressed for salt tolerance of Dunaliella salina . The amplification of mitochondrial DNA might be the result of increasing mitochondria under salt stress.
方孝东,林栖凤,李冠一,屈良鹄. 盐藻线粒体GIY-YIG族归巢内切酶基因受到盐胁迫时增强转录[J]. 中国生物化学与分子生物学报, 2003, 19(05): 625-629.
FANG Xiao dong 1), 2) , LIN Qi feng 2) , LI Guan yi 2) , QU Liang hu 1). Transcription of A Mitochondrial GIY-YIG Family Homing Endonucleases Gene Enhanced in Dunaliella salina Under Salt Stress. Chinese Journal of Biochemistry and Molecular Biol, 2003, 19(05): 625-629.
