Abstract：The Rhizopus arrhizus lipase catalyzed syntheses of three short chain esters in organic solvents were studied. The optimum conditions for each esterification were determined as follows: (1)ethyl caproate: synthesized in cyclohexane at 40℃ with 0 25 mol/L substrate and 1∶1 2 acid alcohol molar ratio; (2)isopropyl acetate: reacted in cyclohexane at 50℃ with 0 15 mol/L substrate and 1∶1 molar ratio; (3)isoamyl acetate: in isooctane at 50℃ with 0 20 mol/L substrate, and 1∶1 molar ratio. 0 125 g per ml of 0 5 nm molecular sieve was required as adsorbent for water in the reactions. Under the optimal conditions, the yields reached 97%—99% after incubation for 8 hours.
杨本宏,吴克,刘斌,郑敏,蔡敬民,潘仁瑞. 非水溶剂中Rhizopus arrhizus脂肪酶催化合成三种酯的最佳条件(英文)[J]. 中国生物化学与分子生物学报, 2003, 19(05): 572-575.
YANG Ben\|hong, WU Ke , LIU Bin, ZHENG Min, CAI Jing\|min, PAN Ren\|rui. Rhizopus arrhizus Lipase Catalyzed Syntheses of Three Esters in Nonaqueous Solvents. Chinese Journal of Biochemistry and Molecular Biol, 2003, 19(05): 572-575.