Abstract：To clone tumor angiogenesis related genes and provide valuable targets for anti angiogenesis, the gene expression profiles between human umbilical vein endothelial cells (HUVEC) activated by tumor homogenates and control HUVEC were compared. To simulate tumor endothelial cells, HUVEC was activated by tissue homogenates from liver and lung cancer. Then suppression subtractive hybridization (SSH) was performed on control HUVEC versus activated HUVEC. The differentially expressed gene fragments were obtained and labeled to screen on the activated HUVEC cDNA library consisting of 4×10 6 recombinants. The false positive clones were picked out by differential hybridization screen. The positive 177 clones were obtained, from which 80 clones were selected randomly and sequenced. Analysis of DNA sequences indicated that positive 74 clones represent 32 genes. Of the 32 genes, several associate with tumor angiogenesis and metastasis as previously reported, one is a novel gene homologous to cytochrome c oxidase Ⅲ, two are novel genes with function unknown, such as KIAA1404, MGC 3067. All these suggest that tumor angiogenesis related genes can be isolated by comparing HUVEC activated by tumor homogenates simulating tumor environment and control HUVEC.
收稿日期: 2005-02-20
引用本文:
刘海燕,胡东,冉宇靓,遇珑,孙立新,刘军,张新宇,杨治华. 抑制消减杂交分离肿瘤血管生成相关基因[J]. 中国生物化学与分子生物学报, 2005, 21(01): 120-127.
LIU Hai Yan,HU Dong,RAN Yu Liang,YU Long,SUN Li Xin,LIU Jun,ZHANG Xin Yu,YANG Zhi Hua *. Isolation of Tumor Angiogenesis Related-Genes by Suppression Subtractive Hybridization. Chinese Journal of Biochemistry and Molecular Biol, 2005, 21(01): 120-127.
