Abstract：Small ubiquitin-like modifier (SUMO) is a recently discovered member of ubiquitin-like protein superfamily that is covalently attached to target proteins as a post-translational modification to alter the localization, stability and/or function of the target protein in response to changes in the cellular environment. To investigate protein SUMOylation, we expressed and affinity purified the human SUMO1. High-titer and specific polyclonal antiserum against human SUMO1 was generated in rabbit, which can be used for identification of SUMO target proteins and studies on biological functions of SUMOylation.
祖玲玲 史颖娇 秦宇 王远根 王照娜 王颖. 类泛素化修饰蛋白SUMO1的表达纯化及抗体制备[J]. 中国生物化学与分子生物学报, 2008, 24(11): 1029-1033.
WANGYing. Overexpression and purification of human SUMO1 and the generation of antibody against human SUMO1. Chinese Journal of Biochemistry and Molecular Biol, 2008, 24(11): 1029-1033.
