Abstract：Fatty acid synthase (FAS) is a potential therapeutic target for obesity and cancer and therefore development of high efficient inhibitors is of vital importance Treatment with 50%ethanol extract of green tea inhibited this enzyme and reduced body weight of rats and mice upon oral administration. The inhibitory activity of the extract onFAS was enhanced markedly in the acidic medium. The increasing inhibitory activity was timedependent by 1 mol/L H2SO4 or HCl at 100℃. Ranging from 25℃to 120℃,increasing temperature raised the speed of enhancement of the inhibitory activity and augment of the final inhibitory capacity. The concentration required for half inhibition of green tea extract decreased by 20 folds and reduced to less than 1 μg/ml by treatment of 1 mol/L HCl in 120℃ for 35 min or 3 mol/L HCl in 100℃ for 30 min. The action by each of four different organic acids with 1 mol/L concentration and pKa value of 4.76 to 1.27 in 100℃ for 150 min enhanced the inhibitory activity of green tea extract to 1.48—5.84 folds, respectively. Higher concentration of hydrogen ion induced more inhibitory effect of the treated extract. The results showed that the speed and degree of the enhancement of inhibitory activity of the extract positively related to the concentration of hydrogen ion in the reaction solution. It is suggested that the enhancement of inhibitory activity of green tea extract in acid roots in some chemical modification of catechins in the extract by hydrogen ion. The primary experiments showed that the reaction was a complicated process. Further investigation is needed to determine the molecular mechanism.
肖文平;张睿;孙颖慧;田维熙. 酸提高绿茶提取物对脂肪酸合酶的抑制活性[J]. 中国生物化学与分子生物学报, 2006, 22(03): 234-234~238.
XIAOWenPing;ZHANGRui;SUNYingHui;TIANWeiXi. Enhancement of Inhibitory Activity of Green Tea Extract on Fatty Acid Synthase by Hydrogen Ion. Chinese Journal of Biochemistry and Molecular Biol, 2006, 22(03): 234-234~238.
