布鲁氏菌ery操纵子参与赤藓醇代谢. 赤藓醇能够促进布鲁氏菌的生长.为进一步研究布鲁氏菌引发宿主流产的分子机制，采用基因重组技术构建布鲁氏菌ery操纵子启动子缺失株（△ery），通过体内外实验探讨布鲁氏菌ery操纵子的生物学功能. 研究结果显示,获得了布鲁氏菌ery操纵子缺失株；布鲁氏菌ery操纵子缺失株侵染胚 胎滋养层细胞脱落较亲本株明显下降；巨噬细胞CFU计数缺失株作用组和亲本株作用组差异显著（P<0.05）.试管凝集和虎红平板实验结果显示均出现凝集现象；检测血清中细胞因子IL-10和TNF-α的表达水平，△ery诱导机体产生的IL-10和TNF-α明显低于亲本株（P<0.05）.小鼠脾脏细菌CFU计数结果显示，△ery较亲本株毒力明显下降.本研究表明，布鲁氏菌ery操纵子启动子缺失株毒力较亲本株明显下降，为进 一步揭示布鲁氏菌引起流产的致病机制提供了一定的理论依据.
Abstract
The ery operon is important for the metabolism of erythritol in Brucella, and erythritol was known to promote the growth of Brucella. To study the molecular mechanisms of Brucella-induced host abortion, an ery operon promoter deletion mutant strain (△ery) was constructed by DNA recombination to explore the biological function of Brucella ery operon in vivo and in vitro. The results showed that the exfoliative human trophoblast cells (HTP-8) infected with the △ery strain was significantly decreased as compared those infected with the wild type in clone forming units (CFU) (P<0.05). In tube agglutination and rose bengal plate agglutination tests, the results showed that the degree of agglutination, as well as the levels of IL-10 and TNF-α were significantly lower in △ery groups (P<0.05) than in the controls. The mouse spleen CFU results showed that the virulence of △ery was significantly decreased. This study provided useful evidence indicating the ery operon could be important for the pathogenesis of Brucella in abortion.
关键词
布鲁氏菌 /
ery操纵子 /
缺失株 /
毒力
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Key words
Brucella /
ery operon /
deletion mutant /
virulence
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中图分类号：
S852
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脚注
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基金
国家重点基础研究发展规划（973项目，No.2010CB30203)；国家自然科学基金(No.31001046/30960288)；高层次人才科研启动资金(No.RCZX200914)
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