脂肪细胞分化是脂肪组织发育的一个重要过程. 目前，鸡脂肪细胞分化的分子调控机制还不十分清楚. 哺乳动物的研究结果表明，转录因子GATA结合蛋白2(Gata2)和GATA结合蛋白3(Gata3)具有抑制脂肪细胞分化的功能，它们在白色脂肪组织和棕色脂肪组织中的表达模式不同. 鸡没有棕色脂肪组织，目前还没有关于Gata2 和Gata3作用于鸡脂肪细胞分化的研究报道. 本研究利用半定量RTPCR的方法分析了Gata2和Gata3基因在鸡腹部脂肪组织和前脂肪细胞中的表达规律，发现鸡腹部脂肪组织中高水平表达Gata2 基因，低水平表达Gata3基因|鸡前脂肪细胞中Gata2 基因的表达水平远高于Gata3基因的表达水平，油酸诱导分化后的鸡前脂肪细胞Gata2基因的表达水平明显下调.此外，鸡过氧化物酶体增殖体激活受体γ(Pparγ)启动子（－1985/－89）报告基因荧光素活性分析和半定量RTPCP发现，在DF1细胞中过表达Gata2 或Gata3抑制鸡PPARγ基因的转录. 本研究结果为进一步研究鸡脂肪细胞分化的分子调控机制和Gata2和Gata3基因的生物学功能提供了参考.
Abstract
Adipocyte differentiation and its regulatory mechanism in chicken adipogenesis remains unclear. The transcription factors GATA binding protein 2(Gata2) and GATA binding protein 3(Gata3), which expressed differently between white and brown adipose tissues, have been shown to inhibit preadipocytes differentiation in mammalians. And it has not reported in chicken, where no brown adipose tissue existed. In the present study, semiquantitative RTPCR analysis indicated that Gata2, but not Gata3, was predominantly expressed in chicken abdominal fat tissues and chicken preadipocytes. The Gata2 expression was significantly reduced in chicken preadipocytes when induced with oleate for differentiation. In addition, the luciferase assay and the semiquantitative RTPCR analysis demonstrated that the overexpression of Gata2 or Gata3 gene in DF1 cells inhibited the gene transcription of the chicken peroxisome proliferatoractivated receptor γ (Pparγ). The results will help to understand the functions of Gata2 and Gata3 in the chicken adipogenesis.
关键词
GATA结合蛋白2(Gata2)｜ GATA结合蛋白3(Gata3)｜ 过氧化物酶体增殖体激活受体&gamma /
（Ppar&gamma /
） 转录
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Key words
GATA binding protein 2(Gata2)｜GATA binding protein 3(Gata3)｜peroxisome proliferatoractivated receptor &gamma /
(Ppar&gamma /
）｜transcription
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中图分类号：
Q75
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参考文献
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脚注
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基金
国家973计划项目课题 (No.2009CB941604 );国家肉鸡产业技术体系建设项目(No.CARS－42);黑龙江省高等学校科技创新团队建设项目(No.2010td02)
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