Department of Physiology, Key Laboratory for Neurodegenerative Disorders of Ministry of Education, Capital Medical University, Beijing 100069, China
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文章历史+
收稿日期
修回日期
出版日期
2009-12-03
2010-12-27
2011-03-20
发布日期
2011-03-24
摘要
细胞内的RNA一般不会单独存在,而是与各种各样的RNA结合蛋白(RBPs)绑定在一起,形成核糖核蛋白复合体(RNP complexes)影响着RNA的加工与转归. Poly(C)结合蛋白是一类重要的RNA结合蛋白,可分为两组:hnRNP K 和PCBP14. 它们以序列特异的方式与核酸嘧啶富含区相结合. 这类蛋白具有共同的结构模体(motif),即hnRNP K 同源(KH)域. KH域是与mRNA结合的结构基础,也是机体内调控系统的组成部分,可使得Poly(C)结合蛋白参与蛋白/核酸、蛋白/蛋白之间的相互作用,范围涉及复制、转录、mRNA稳定和翻译控制过程等. 对Poly(C)结合蛋白功能的深刻认识可使我们洞察多种疾病的病理生理过程.
Abstract
The processing and ultimately turnover of mRNA are complex processes that are highly regulated. Within a cell, RNA is usually not in a naked form. It forms ribonucleoprotein (RNP) complexes with various RNAbinding proteins (RBPs), thereby influencing processing and turnover events. Poly(C)binding proteins (PCBPs), generally known as RBPs, interact in a sequencespecific fashion with singlestranded poly(C). They can be divided into two groups: hnRNP K and PCBP14. PCBPs and hnRNP K share a common structural motif, the hnRNP K homology (KH) domain, which provides a structural basis for mRNA binding. The KHdomains are components of a modular system, which enables the proteins to be engaged in both protein/nucleic acid and protein/protein interactions. The latter interactions are involved in cell signaling events. As components of different mRNAprotein complexes, PCBPs and hnRNP K have been identified as factors involved in regulating duplication, transcription, mRNA stability and translation. They have also been found related to some pathological processes, such as HutchinsonGilford Progeria syndrome, virus infections and malignancy.