SIRT1影响Tat介导的HIV-1转录

桑伟伟, 张红胜

中国生物化学与分子生物学报 ›› 2010, Vol. 26 ›› Issue (11) : 975-980.

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PDF(324 KB)
中国生物化学与分子生物学报 ›› 2010, Vol. 26 ›› Issue (11) : 975-980.
综述

SIRT1影响Tat介导的HIV-1转录

  • 桑伟伟,张红胜*
作者信息 +

Effect of SIRT1 on the transcription of HIV-1 induced by Tat

  • SANG Wei-Wei, ZHANG Hong-Sheng*
Author information +
文章历史 +

摘要

 Tat蛋白在HIV的转录复制中起重要作用.它能反式激活HIV的转录,促进HIV长末端重复序列(HIV LTR)的转录和延长. Tat蛋白是去乙酰化酶SIRT1的一种重要底物.Tat的乙酰化与非乙酰化状态在激活转录过程中受高度精密调控.如果Tat乙酰化状态在转录过程中受到干扰,随后其促使的HIV转录也将受到干扰.近来发现,组蛋白去乙酰化酶SIRT1在Tat蛋白介导的反式激活HIV转录过程中起重要的调控作用.SIRT1能对乙酰化的Tat进行去乙酰化,使其能在促使HIV转录的过程中循环利用.同时Tat与SIRT1的结合也会使核转录因子NF-κB的p65亚基处于超乙酰化状态,致使病毒基因组表达.研究SIRT1与Tat的相互关系为治疗HIV提供了新的方向.

Abstract

Tat protein plays an important role in HIV transcription and replication, which can trans-activate HIV transcription. Transcription of human immunodeficiency virus (HIV-1) is activated by viral Tat protein which regulates the transcription and elongation of HIV long terminal repeat (LTR). HIV-1 Tat protein is a substrate for the deacetylase sirtuin 1 (SIRT1). The status of Tat, whether it is acetylated or not in the transcription process, is highly precise controlled. If the acetylation state of Tat was interfered in the transcription process, HIV transcription could also be subject to be interfered. Recent study showed that histone deacetylase SIRT1 protein regulates Tat-mediated trans-activation of HIV transcription. SIRT1 could deacetylate acetylated Tat, thus it can facilitate the process of HIV transcription recycling. The combination of Tat and SIRT1 can also make the p65 subunit of nuclear transcription factor NF-κB be in super-acetylation state, resulting in the expression of virus genome. The relationship between SIRT1 and the Tat provides a new direction for the treatment of HIV.

关键词

 

 
/ Tat / 去乙酰化酶SIRT1 / 人类免疫缺陷病毒(HIV) / NF-&kappa / B Tat / SIRT1 / HIV / NF-κB

Key words

 

 Tat
/ deacetylase sirtuin 1(SIRT1) / human immunodeficiency virus (HIV) / NF-κB Tat / SIRT1 / HIV / NF-κB

引用本文

导出引用
桑伟伟, 张红胜. SIRT1影响Tat介导的HIV-1转录[J]. 中国生物化学与分子生物学报, 2010, 26(11): 975-980
SANG Wei-Wei, ZHANG Hong-Sheng. Effect of SIRT1 on the transcription of HIV-1 induced by Tat[J]. Chinese Journal of Biochemistry and Molecular Biology, 2010, 26(11): 975-980
中图分类号: Q78   

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基金

国家自然科学基金项目;国家“九七三”计划项目

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