红海榄根部盐胁迫反应的比较蛋白质组学分析

范吉星1,,邓用川1,,黄惜1,,,林栖凤1,,罗越华1,

中国生物化学与分子生物学报 ›› 2009, Vol. 25 ›› Issue (01) : 72-72~77.

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中国生物化学与分子生物学报 ›› 2009, Vol. 25 ›› Issue (01) : 72-72~77.
研究论文

红海榄根部盐胁迫反应的比较蛋白质组学分析

  • 范吉星1),3),邓用川1),2),黄惜1),2),3),林栖凤1),2),罗越华1),3)*
作者信息 +

Comparative Proteomic Analysis of Salt-stress Response Proteins in Rhizophora StylosanRoots

  • FAN Ji-Xing1),3)**, DENG Yong-Chuan1),2)**, HUANG Xi1), 2),3), LIN Qi-Feng1), 2), LUO Yue-Hua1),3)*
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摘要

红海榄(Rhizophora stylosa)是一种典型的红树林盐生植物.本研究利用蛋白组学技术对淡栽(R0)和3% NaCl盐栽(R3)处理后的红海榄根部总蛋白进行了比较研究.双向电泳图谱的结果表明,R0和R3分别有981和972个蛋白点,蛋白点主要集中在分子量28~70 kD,等电点4.0~8.5之间. R0和R3之间差异明显的有15个蛋白点.其中,8个蛋白的表达量在R0中表达增高(10倍),而在R3中相对下降.另外,7个蛋白的表达量在R0中较低,而在R3中表达量显著增高.对这15个蛋白点进行肽质量指纹图谱分析,10个蛋白点找到匹配蛋白.功能预测分析发现,在盐水栽培上调的蛋白质一般与逆境胁迫有关,淡水栽培上调的蛋白质一般与基本代谢有关.这些研究结果为进一步研究红海榄的耐盐机理提供了有意义的线索.

Abstract

Rhizophora stylosa is a typical halophyte in mangrove forests. In this study, we analyzed the differential expression of salt-stress responsive proteins in the roots of Rhizophora stylosatreated with fresh water (R0) or 3% NaCl solution (R3). Two-dimensional electrophoresis (2-DE) showed that 981 and 972 protein spots were identified in respect to different conditions, and most proteins with pI 4.0—8.5 and molecular weight ranged at 28—70 kD. 15 proteins were found to be differentially expressed in R0 and R3, 8 of which were up-regulated (10 fold) in R0, and 7 in R3. MALDI-TOF-MS analysis showed that 10 out of the 15 proteins could be identified through peptide mass fingerprint (PMF). The proteins up-regulated in R3 were associated with the stressinduction, whereas those highly expressed in R0 were related to the basic metabolism. These results may provide important information to understand the molecular mechanisms of salt-tolerant in Rhizophora stylosa.

关键词

红海榄 / 盐胁迫反应 / 比较蛋白组学 / 双向电泳 / 质谱

Key words

Rhizophora stylosa / salt-stress response / comparative proteomic analysis / two-dimensional electrophoresis (2-DE) / mass spectrometry

引用本文

导出引用
范吉星1,,邓用川1,,黄惜1,,,林栖凤1,,罗越华1,. 红海榄根部盐胁迫反应的比较蛋白质组学分析[J]. 中国生物化学与分子生物学报, 2009, 25(01): 72-72~77
FANJi-Xing,,DENGYong-Chuan,,HUANGXi,,,LINQi-Feng,,LUOYue-Hua,. Comparative Proteomic Analysis of Salt-stress Response Proteins in Rhizophora StylosanRoots[J]. Chinese Journal of Biochemistry and Molecular Biology, 2009, 25(01): 72-72~77
中图分类号: Q71    Q816   

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