甲硫氨酸脑啡肽对猴免疫缺陷病毒早期感染引起CEM x1 74细胞凋亡的可能作用(英文)

PDF(546 KB)

中国生物化学与分子生物学报 ›› 2003, Vol. 19 ›› Issue (03) : 305-311.

论文

甲硫氨酸脑啡肽对猴免疫缺陷病毒早期感染引起CEM x1 74细胞凋亡的可能作用(英文)

郝艳生,张奉学,黄冬爱,刘新华,李平风,李刚

作者信息 +

Possible Effect Pathway of Methionine Enkephalin on Apoptosis of CEM x174 Cells Induced by Simian Immunodeficiency Virus in Early Stages of Infection

HAO Yan sheng 1) , ZHANG Feng xue 2) , HUANG Dong ai 3) , LIU Xin hua 1) , LI Ping feng 1)* , LI Gang 1)*

Author information +

文章历史 +

摘要

探讨短期甲硫氨酸脑啡肽作用对SIV感染CEMx174细胞凋亡的可能作用 .用MTS法测定甲硫氨酸脑啡肽对感染CEMx174细胞存活率的影响 ,流式细胞仪分析SIV诱导细胞凋亡及其甲硫氨酸脑啡肽的作用 ,并测定了cAMP含量、PKA活性和组蛋白磷酸化的水平 .SIV能够显著减少CEMx174细胞数 ,甲硫氨酸脑啡肽可以提高感染细胞的存活率 .AnnexinⅤ结合实验显示 ,1μmol L甲硫氨酸脑啡肽可以增加存活细胞的比率 ,减少凋亡细胞数 .甲硫氨酸脑啡肽降低正常细胞和感染细胞cAMP的含量和PKA活性 ,但是在感染组较为明显 .在感染的情况下 ,磷酸化的组蛋白增加 ,甲硫氨酸脑啡肽可以减少其含量 .甲硫氨酸脑啡肽的作用可以被纳酪酮所拮抗 .研究结果提示 ,甲硫氨酸脑啡肽对SIV感染引起早期细胞凋亡的作用涉及cAMP PKA信号传递过程

Abstract

The possible mechanism of apoptosis alleviated by short term exposure of methionine enkephalin to CEM x174 cells infected with SIV was elucidated. The effects of methionine enkephalin on the viability of normal CEM x174 cells and infected cells were determined by the method of MTS assay. The apoptosis induced by SIV and the effects of methionine enkephalin were analyzed by flow cytometry. The levels of cAMP, the activities of PKA and the levels of phosphorylation of histone H3 were measured.A pronounced decrease of the cell number in SIV infected group could be noticed compared with control. Methionine enkephalin could elevate the viability of cells in infected groups. The results exhibited that Methionine enkephalin at the concentrations of 1 μmol/L could increase the percentage of vital cells and decrease the apoptotic cells based on annexin Ⅴ binding assay. Methionine enkephalin could also downregulate the content of cAMP and the activity of PKA in both groups, but more obvious in SIV infected group. The phosphorylated histone H3 was elevated in the case of virus infection and quantitatively decreased in the presence of methionine enkephalin. All effects of methionine enkephalin could be blocked by naloxone. The results indicated that cAMP PKA signal transduction cascade was involved in the regulation of methionine enkephalin on apoptosis induced by SIV during early stages.

导出引用

郝艳生,张奉学,黄冬爱,刘新华,李平风,李刚. 甲硫氨酸脑啡肽对猴免疫缺陷病毒早期感染引起CEM x1 74细胞凋亡的可能作用(英文)[J]. 中国生物化学与分子生物学报, 2003, 19(03): 305-311

HAO Yan sheng 1) , ZHANG Feng xue 2) , HUANG Dong ai 3) , LIU Xin hua 1) , LI Ping feng 1)* , LI Gang 1)*. Possible Effect Pathway of Methionine Enkephalin on Apoptosis of CEM x174 Cells Induced by Simian Immunodeficiency Virus in Early Stages of Infection[J]. Chinese Journal of Biochemistry and Molecular Biology, 2003, 19(03): 305-311

参考文献

1　PetersonPK ,MolitorTW ,ChaoCC .Theopioid cytokineconnection.JNeuroimmunol,1998,83:63～69 2　PlotnikoffNP ,FaithRE ,MurgoAJ,HerbermanRB ,GoodR A .Methionineenkephalin:anewcytokine—humanstudies.ClinImmunolImmunopathol,1997,82(2):93～101 3　LiG ,FrakerPJ.Methionineenkephalinalterationofmitogenicandmixedlymphocytecultureresponsesinzinc deficientmice.ActaPharmacolSin,1989,10(3):216～221 4　LiG ,YuJ.InhibitionofbonemarrowimmatureBlymphocytesfromzincdeficientmicebymethionineenkephalin.ActaPharmacolSin,1991,12(6):500～503 5　LiG ,YuJ,HouJZ .Regulationofmethionineenkephalinonthefunctionoflymphocytesandmacrophagesformliver impairedmice.ChinJPharmacolToxicol,1997,11(3):180～183 6　LiG ,YangFY ,LiuXH ,WangD ,LiPF .Apossiblemechanismofapoptosisinducedbysimianimmunodeficiencyvirusinpolymorphonuclearneutrophils.VirolSin,2001,16(4):304～308 7　MeyaardL ,OttoAS ,KeetJPM ,RoosMTL ,MiedemaF .ProgrammeddeathofTcellsinhumanimmunodeficiencyvirusinfection.Nocorrelationwithprogressiontodisease.JClinInvestig,1994,93:982～988 8　MischiatiC ,PironiF ,MilaniD ,Giacca,M ,MirandolaP ,CapitaniS ,ZauliG .ExtracellularHIV 1TatproteindifferentiallyactivatestheJNKandERK MAPKpathwayinCD4Tcell.AIDS ,1999,13:1637～1645 9　NaritaM ,MizoguchiH ,KampineJP ,TsengLF .RoleofproteinkinaseCindesensitizationofspinaldelta opioid mediatedantinociceptioninthemouse.BrJPharmacol,1996,118(7):1829～1835 10　ShenJ,BenedictGA ,GallagherA ,StaffordK ,YoburnBC .Roleofcamp dependentproteinkinase(PKA )inopioidogonist induced&mgr, opioidreceptordownregulationandtoleranceinmice.Synapse,2000,38(3):322～327 11　AvidorReissT ,BayewitchM ,LevyR ,MatusLeibovitchN ,NevoI,VogelZ .Adenylylcyclasesupersensitizationinmu opioidreceptor transfectedChinesehamsterovarycellsfollowingchronicopioidtreatment.JBiolChem,1995,270(50):29732～29738 12　LiuJG ,AnandKJ .Proteinkinasesmodulatethecellularadaptationsassociatedwithopioidtoleranceanddependence.BrainResBrainResRev,2001,38(12):1～19 13　WagnerEJ,RonnekleivOK ,KellyMJ .ProteinkinaseAmaintainscellulartolerancetomuopioidreceptoragonistsinhypothalamicneurosecretorycellswithchronicmorphinetreatment:convergenceonacommonpathwaywithestrogeninmodulatingmuopioidreceptor effectorcoupling.JPharmacolExpTher,1998,285(3):1266～1273 14　LiMS ,LiPF ,HeSP ,DuGG ,LiG .Theintracellularmechanismofalpha fetoproteinpromotingtheproliferationofNIH 3T3cells.CellRes,2002,12(2):151～156 15　LiMS ,LiPF ,HeSP ,DuGG ,LiG .Thepromotingmolecularmechanismofalpha fetoproteinonthegrowthofhumanhepatomaBel7402cellline.WorldJGanstroenterol,2002,8(3):469～475 16　ChuangLF ,KillamJrKF ,ChuangRY .IncreasedreplicationofsimianimmunodeficiencyvirusinCEMx174cellsbymorphinesulfate.BiochemBiophyResCommun,1993,195(3):1165～1173 17　李刚,LamKM .阿片类物质对猴免疫缺陷病毒感染的CEMX174细胞内p53合成的调节作用.中国生物化学与分子生物学报(LiG ,LamKM .Regulationofopiatesonsynthesisofintracellularproteinp53inCEMx174cellsinfectedwithsimianimmunodeficiencyvirus.ChinJBiochemMolBiol),2000,16(2):162～168 18　黄冬爱,李刚.淋巴细胞存在有δ阿片肽受体的分子证据.中国生物化学与分子生物学报(HuangDong ai,LiGang.Molecularproofofδopioidpeptidereceptoronlymphocytes.ChinJBiochemMolBiol),1998,14(5):503～505 19　黄冬爱,杨非易,李平风,李刚,葛韵琴,杜国光.甲硫氨酸脑啡肽和抗阿片肽δ受体抗体对小鼠骨髓瘤细胞信号传递系统的作用.中国生物化学与分子生物学报(HuangDong ai,YangFei yi,LiPing feng,LiGang,GeYun qin,DuGuo guang.Theroleofmethionine enkephalinandantibodyagainstopioidrecptoronsignaltransductionsysteminmouseNS 1cells.ChinJBichemMolBiol),2000,16(5):660～664 20　LiapiC ,TakahashiN ,RaynaudF ,EvainBrionD ,AndersonWB .Effectsof[D Ala1]peptideT NH2andHIVenvelopeglycoproteingp120oncyclicAMPdependentproteinkinasesinnormalandpsoriatichumanfibroblasts.JInvestDermatol,1998,110(4):332～337 21　ZidovetzkiR ,WangJL ,ChenP ,JeyaseelanR ,HofmanF .HumanimmunodeficiencyvirusTatproteininducesinterleukin6mRNAexpressioninhumanbrainendothelialcellsviaproteinkinaseCandcamp dependentproteinkinasepathways.AIDSResHumRetroviruses,1998,14(10):825～833 22　ShapiraM ,GafniM ,SarneY .Independenceof,andinteractionbetween,cannabinoida

PDF(546 KB)

Accesses

Citation

Detail

段落导航

摘要
Abstract
关键词
Key words
引用本文
参考文献

收稿日期	修回日期	出版日期
2003-06-20	2003-06-20	2003-06-20
发布日期
2003-06-20

选择文件类型/文献管理软件名称

选择包含的内容

摘要

Abstract

关键词

Key words

引用本文

{{custom_sec.title}}

{{custom_sec.title}}

参考文献

{{custom_fnGroup.title_cn}}

脚注

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

摘要

Abstract

关键词

Key words

引用本文

{{custom_sec.title}}

{{custom_sec.title}}

参考文献

{{custom_fnGroup.title_cn}}

脚注