以端粒酶为靶标抗癌药物筛选模型建立及端粒酶抑制剂筛选

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中国生物化学与分子生物学报 ›› 2002, Vol. 18 ›› Issue (01) : 125-128.

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以端粒酶为靶标抗癌药物筛选模型建立及端粒酶抑制剂筛选

郑晓飞,王升启,孙志贤

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Determination of Telomerase from HeLa Cells as a Target for Screening Antitumor Agents

ZHENG Xiao\|fei , WANG Sheng\|qi, SUN Zhi\|xian

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Abstract

Telomerase, a ribonucleoprotein enzyme, has been found in immortalized but not in most somatic adult human tissues, and thus emerged as a novel target for cancer chemotherapy. Recently it has been found that telomerase is a fruitful target for oncologic drug development. A new method for screening antitumor agents by using telomerase as a target has been established according to the phenomena that the enzyme activity can be affected by some types of antitumor agents or chemicals. The telomerase was extracted from HeLa cells. The telomeric repeat amplification protocol(TRAP) was used to measure enzyme activity. Telomerase activity can be inhibited by 4 kinds of chemical compounds.

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郑晓飞,王升启,孙志贤. 以端粒酶为靶标抗癌药物筛选模型建立及端粒酶抑制剂筛选[J]. 中国生物化学与分子生物学报, 2002, 18(01): 125-128

ZHENG Xiao\|fei , WANG Sheng\|qi, SUN Zhi\|xian. Determination of Telomerase from HeLa Cells as a Target for Screening Antitumor Agents[J]. Chinese Journal of Biochemistry and Molecular Biology, 2002, 18(01): 125-128

参考文献

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收稿日期	修回日期	出版日期
2002-02-20	2002-02-20	2002-02-20
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2002-02-20

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