HFRSV内影像型抗独特型人单抗重链可变区基因克隆

高磊,陈苏民,陈南春,赵忠良,刘新平,杨萍,杨安钢,米力,崔运昌

中国生物化学与分子生物学报 ›› 1994, Vol. 10 ›› Issue (03) : 274-278.

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中国生物化学与分子生物学报 ›› 1994, Vol. 10 ›› Issue (03) : 274-278.
论文

HFRSV内影像型抗独特型人单抗重链可变区基因克隆

  • 高磊,陈苏民,陈南春,赵忠良,刘新平,杨萍,杨安钢,米力,崔运昌
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Gene Cloning of the Heavy-Chain Variable Region of an Internal Image Human Monoclonal Anti-idiotype Antibody against Hemorrhagic Fever Virus

  • Gao,Lei; Chen,Su-min; Chen,Nan-chun; Zhao,Zhong-ling; Liu,Xinping; Yang,Ping;Yang,An-gang; Mi,Li; Cui,Yun-chang
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摘要

利用反转录-PCR方法,从分泌肾综合症出血热病毒(HFRSV)内影像型抗独特型人单抗杂交瘤细胞系(C8)中,成功地克隆了抗HFRSV1d人单抗重链可变区基因,并将此基因重组入M13噬菌体DNA中,测定了此重链可变区基因全序列。经计算机分析,基因全长共351bp、编码117个氨基酸,氨基酸序列同源性分析发现所推得的该单抗CDR2区与HFRSVG2蛋白C端有同源区,此区可能具有较强的抗原性。

Abstract

The heavy-chain variable region of an internal image human monoclonal antiidiotype antibody against Hemorrhagic Fever Virus was cloned from the hybridoma cell line (C8) by Reverse transcription-PCR method. The nucleotide sequence of the amplified DNA was determined after subcloning of PCR fragment in M13 vector. By computer analysis, the VH region consists of 351 base pairs encoding 117 amino acids. The comparison of amino acid sequences between the deduced CS VH peptide and HFRSV proteins revealed high homology between the CDRZ of the VH and the GZ of HFRSV. The antigenicity of this region was discussed.

关键词

抗独特型抗体;PCR;可变区基因;肾综合症出血热病毒(HFRSV)

Key words

Anti-idiotype antibody / RCR / Variable region / HFRSV

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高磊,陈苏民,陈南春,赵忠良,刘新平,杨萍,杨安钢,米力,崔运昌. HFRSV内影像型抗独特型人单抗重链可变区基因克隆[J]. 中国生物化学与分子生物学报, 1994, 10(03): 274-278
Gao,Lei; Chen,Su-min; Chen,Nan-chun; Zhao,Zhong-ling; Liu,Xinping; Yang,Ping;Yang,An-gang; Mi,Li; Cui,Yun-chang. Gene Cloning of the Heavy-Chain Variable Region of an Internal Image Human Monoclonal Anti-idiotype Antibody against Hemorrhagic Fever Virus[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(03): 274-278

参考文献

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