α淀粉酶与糖化酶的共固定化研究

郭桥,罗贵民,孙启安,黄仲立,马林

中国生物化学与分子生物学报 ›› 1994, Vol. 10 ›› Issue (03) : 259-263.

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PDF(310 KB)
中国生物化学与分子生物学报 ›› 1994, Vol. 10 ›› Issue (03) : 259-263.
论文

α淀粉酶与糖化酶的共固定化研究

  • 郭桥,罗贵民,孙启安,黄仲立,马林
作者信息 +

Studies on Coimmobilization of α-amylase and Glucoamylase

  • Guo,Qiao; Luo,Gui-min; Sun,Qi-an; Huang,Zhong-li; Ma,Lin
Author information +
文章历史 +

摘要

以纤维素为载体用重氮化法同时固定了糖化酶和α淀粉酶,确定了共固定化的最适条件,研究了共固定化酶的性质,发现共固定化酶较固定化单酶能更好地发挥协同效应,能在较低温度下将淀粉一步水解为葡萄糖。共固定化酶在30℃下的操作半寿期可达920小时。

Abstract

Glucoamylase and α-amylase were simultaneously immobilized on cellulose by using diazo method. The optimal conditions for coimmobilization were established, and the properties of the coimmobilized enzymes were studied. It was found that coimmobilized enzyme has better synergism compared with separately immobilized enzymes, and starch can be directly converted into glucose under lower temperature by coimrnobilized enzymes. The half life of the coimmobilized enzymes operated at 30℃ is more than 920h.

关键词

糖化酶;α淀粉酶;共固定化酶;协同效应;纤维素

Key words

Glucoamylase / α-amylase / Coimmobilized enzymes

引用本文

导出引用
郭桥,罗贵民,孙启安,黄仲立,马林. α淀粉酶与糖化酶的共固定化研究[J]. 中国生物化学与分子生物学报, 1994, 10(03): 259-263
Guo,Qiao; Luo,Gui-min; Sun,Qi-an; Huang,Zhong-li; Ma,Lin. Studies on Coimmobilization of α-amylase and Glucoamylase[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(03): 259-263

参考文献

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