Glucoamylase and α-amylase were simultaneously immobilized on cellulose by using diazo method. The optimal conditions for coimmobilization were established, and the properties of the coimmobilized enzymes were studied. It was found that coimmobilized enzyme has better synergism compared with separately immobilized enzymes, and starch can be directly converted into glucose under lower temperature by coimrnobilized enzymes. The half life of the coimmobilized enzymes operated at 30℃ is more than 920h.
Guo,Qiao; Luo,Gui-min; Sun,Qi-an; Huang,Zhong-li; Ma,Lin.
Studies on Coimmobilization of α-amylase and Glucoamylase[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(03): 259-263