Abstract：Decorsin cDNA was obtained and inserted into expression vector pMAL\|p2x by DNA recombinant method,which was checked by nucleotide sequencing.The fusion protein of MBP\|decorsin was produced by IPTG induction in E.coli (TB1).The expression level was approximately 8%.The fusion protein was purified by affinity column,showing one main protein band in SDS\|PGE analysis.The platelet aggregation experiment revealed that the fusion protein had the activity of anti\|platelet with IC 50 value of 370 nmol/L.
焦建伟,张磊亮,俞梅敏,茹炳根. 抗栓肽(Decorsin)在大肠杆菌中的克隆及表达[J]. 中国生物化学与分子生物学报, 2001, 17(05): 602-605.
JIAO Jian\|wei,ZHANG Lei\|liang, YU Mei\|min, RU Bing\|gen *. Cloning and Expression of an Antithrombolytic Peptide Decorsin in E.coli. Chinese Journal of Biochemistry and Molecular Biol, 2001, 17(05): 602-605.
