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�й����ﻯѧ���������ѧ�� 1987, Vol. 3 Issue (03 ):279-279     DOI:
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STUDY EPITOPES OF HUMAN CHORIONIC GONADOTROPIN BY MONOCLONAL ANTIBODIES AND PROTEOLYTIC ENZYMES
Shan,Li-xin Huang, Bao-shan (Department of biochemistry, Beijing Medical University) Li, Wei-xiong (Department of Physiology, Beijing Medical University)

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ժҪ ���ı���,�ڲ�ͬø��������,�����Ⱦ��ø���ӵ���ø���ȵ���ø,ø������ëĤ�����ټ���(hCG)���ĸ�Ƭ��,����θ����ø������Ƭ��;��Щø��Ƭ���ھ۱�ϩ�������Ӿ�е�����λ�ò�ͬ,˵��Ϊ�Ǿ�һ��ø����Ӧ�õ�����ת�Ƶ�Ӿ��������������ά��Ĥ�Ͻ�������ø��Ⱦɫ,������hCG�ĵ���¡����9C_2��8B_5��������hCG������hCGø��Ƭ�η�Ӧ;��7F_3��BAH��10E_8ֻ������hCG��Ӧ������hCG�����п��ܴ��������ͺͽṹ�͵����ֲ�ͬ�Ŀ�ԭ�����ء�
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Abstract�� Four fractions have been obtained from hydrolysis of human chorionic gona-dotropin (hCG) with thermolysin, trypsin or chymotrypsin, and three fractions are produced by pepsin digestion. These fractions migrate to form separate bands at different positions on polyacrylamide gel electrophoresis (PAGE) showing that these fractions are non-homogeneous. By using the Western Blot technique and immunoperoxidase staining on nitrocellulose sheet, we have identified 2 anti-hCG monoclonal antibodies (McAb) 9C2 and 8B5 reactive to intact hCG and all of these fractions, whereas McAb 7F3, BAH1, 10E8 can only react with intact hCG. The result suggest that there are at least two classes of epitopes in hCG.
Keywords�� Human Chorionic Gonadotropin   Monoclonal Antibody   Proteolytic Enzymes   Western Blot.      
Received 1986-06-16;
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������.�õ���¡����͵���ˮ��ø�о�����ëĤ�����ټ��صĿ�ԭ������ [J]  �й����ﻯѧ���������ѧ��, 1987,V3(03 ): 279-279
.STUDY EPITOPES OF HUMAN CHORIONIC GONADOTROPIN BY MONOCLONAL ANTIBODIES AND PROTEOLYTIC ENZYMES [J]  Chinese Journal of Biochemistry and Molecular Biol, 1987,V3(03 ): 279-279
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http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y1987/V3/I03 /279