首 页期刊介绍编 委 会征稿简则优秀论文出版伦理期刊订阅广告合作下载中心联系我们 English
�й����ﻯѧ���������ѧ�� 1989, Vol. 5 Issue (04 ):294-294     DOI:
���� ����Ŀ¼ | ����Ŀ¼ | ����� | �߼����� << [an error occurred while processing this directive] | [an error occurred while processing this directive] >>
��̥�̹��׸���S-ת��ø����ѧ�����Ƽ����о�
�ij��� *
@�ij���$�Ϻ�ҽ�ƴ�ѧ���ﻯѧ������!�Ϻ� @�»���$�Ϻ�ҽ�ƴ�ѧ���ﻯѧ������!�Ϻ�
Studies on the Kinetics and Inhibitors Of Human Placenta Glutathione S-Transferase
Xia, Chu-lin Chen, Hui-li (Department of Biochemistry, Shanghai Medical University, Shanghai)

ժҪ
�����
�������
Download: PDF (1328KB)   HTML 0KB   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ �о�����̥���͹��׸���S-ת��ø(GST-��)�Ķ���ѧ������GSH��1-��-2,4-������(CDNB)��km�ֱ�Ϊ0.109��0.870mmol/L��������ù�غ��ȷ�ù�آ�������GST����,���ȷ�ù�ؽ�����,���Ǿ��������ơ�����K��CDNBҲ�ǷǾ�������,�����������CDNB������GSH�Ǿ��������ø������S-�������S-��������׸�����GSH�������CDNB�Ǿ��������GST-�С��ѳ��֤��GST-����߻���˫���ﷴӦ�����˳����ơ���ѧ����ʵ�鷢��:�ϻ��һ����Ȼ���������ܲ���ø�������ĵ���ɡ�
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
�ij���
�ؼ���� ��̥���͹��׸���S-ת��ø(GST-��)   �����Ի�Ǿ���������   ���˳�����   ��ѧ����     
Abstract�� The kinetics and inhibitors of purified glutathione S-transf erase from-human placenta (GST-��) were studied. The Km of substrate glutathione(GSH) and 1-chlore-2,4-dinitrobenzene (CDNB) were 0.109 and 0.870 m mol/L respectively, Oxacillin and cephalothin were shown to inhnbit GST-��, and inhibition with cephalothin was more obvious and noncompetitive. Bromosulfophthalein was also noncompetitive inhibitor with respect to CDNB, whereas bilirubin was competitive with CDNB and noncompetitive with GSH in inhibiting GST-��. Two derivatives of GSH, S-n-octy-lglutathione and S-n-hexylglutathione, were competitively with GSH and noncompe-titively with CDNB to inhibit GST-��. It was well proved that the mechanism of GST-�� catalyzed reaction was random sequential. Chemical modification studies showed that the sulfhydryl, guanido, amino, carboxyl and indolyl groups may par ticipate in the active center of the enzyme.
Keywords�� Human placenta type glutathione S-transferase (GST-��) Competitive or noncompetitive inhibition Random sequential mechanism Chemical modification     
Received 1987-10-26;
���ñ���:   
�ij���.��̥�̹��׸���S-ת��ø����ѧ�����Ƽ����о� [J]  �й����ﻯѧ���������ѧ��, 1989,V5(04 ): 294-294
.Studies on the Kinetics and Inhibitors Of Human Placenta Glutathione S-Transferase [J]  Chinese Journal of Biochemistry and Molecular Biol, 1989,V5(04 ): 294-294
���ӱ���:  
http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y1989/V5/I04 /294
Copyright 2010 by �й����ﻯѧ���������ѧ��