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�й����ﻯѧ���������ѧ�� 1997, Vol. 13 Issue (03 ):308-308     DOI:
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Physicochemical Properties and Secondary Structure Prediction of Buzura suppressaria Nuclear Polyhedrosis Virus Polyhedrin
Chen Hua; Chen Dong; Gong Wei-Min; Teng Mai-Kun; Zhu Xue-Yong; Niu Li-Men (Young Scientist Laboratory of Structure Biology and Department of Biology,University of Science and Technology of China, Hefei 230026)

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Abstract�� Using SDS-PAGE and gel concentration gradient electrophoresis,It was determined that the molecular weights of Buzura suppressaria nuclear polyhedrosis virus (BsNPV) polyhedrin and its subunit are 363 kD and 31. 5 kD respectively and this protein was composed of twelve subunits without disulfide bonds among the subunits. The circular dichroism (CD) spectrum indicated that the secondary structure of polyhedrin consisted of 31, 7 % ��-helix, 23. 8% ��sheet,and 44. 5 % random-coil,which was in coincidence with the result of prediction. The fluoresence studies of polyhedrin showed that the surface hydrophobicity of polyhedrin was weak and the tryptophan residues in each subunit were in the internal hydrophobic region.
Keywords�� Buzura suppressaria nuclear polyhedrosis virus   Polyhedrin   Circular dichroism   Fluorescence   Prediction of secondary structure      
Received 1996-04-15;
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�»�.��ͩ�����Ͷ���岡������嵰�׵��?���ʼ������ṹԤ�� [J]  �й����ﻯѧ���������ѧ��, 1997,V13(03 ): 308-308
.Physicochemical Properties and Secondary Structure Prediction of Buzura suppressaria Nuclear Polyhedrosis Virus Polyhedrin [J]  Chinese Journal of Biochemistry and Molecular Biol, 1997,V13(03 ): 308-308
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http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y1997/V13/I03 /308