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�й����ﻯѧ���������ѧ�� 2000, Vol. 16 Issue (04 ):499-499     DOI:
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Electronic Absorption Spectroscopy Properties of Soybean Hull Peroxidase
LIU Wen,ZHANG Hua,QI Fei,GAO Pei ji,FANG Jing (State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China)

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ժҪ Ӧ�õ������չ��׼����о��˶��ǹ�������ø ( EC1 .1 1 .1 .7)�IJ�ͬ����̬�������չ��� ,����������Դ�Ĺ�������ø���˱Ƚ��о� .��Ȼ̬ø���������շ�λΪ 40 4 nm�� Soret�� ,638nm�Ħ���� 50 8nm�Ħ´� ,��������ⷴӦ��������ิ���� .������ ( Com )�� 40 8��580��61 8�� 655nm�������������� ;������ ( Com )�� 41 9��52 9�� 556nm����ʾ�������� ;������ ( Com )���� 41 8��543�� 578nm����ʾ�������� .��Ȼ̬ø�������������ƻ�ԭ����� 435�� 558nm�������� ,���軯�������� 42 2�� 544nm����ʾ�������� .�軯�ضԸ�ø������Ϊ���������� ,�� Ki ֵΪ 2 .4��mol/L.
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Abstract�� The hydrogen peroxide oxidized forms of soybean hull peroxidase compounds ��,�� and �� were characterized by electronic absorption spectroscopy.The native enzyme had its Soret maximum at 404 nm and �� and �� bands at 638 and 508 nm.Addition of 1 molar equiv.of hydrogen peroxide to the native enzyme yielded compound ��,characterized by a Soret maximum at 408 nm with reduced intensity and by additional maxima at 580,618,and 655 nm.Addition of 2 molar equiv.of hydrogen peroxide to native SHP yielded compound ��,identified by absorption maxima at 419,529,and 556 nm.Addition of a molar excess of hydrogen peroxide to the native enzyme yielded compound ��,characterized by absorption maxima at 418,543 and 578 nm.Addition of sodium dithionite yielded the reduced form of the enzyme,characterized by absorption maxima at 435 and 558 nm.Addition of cyanide immediately changed the spectrum of native SHP and produced cyanide SHP compound,identified by absorption maxima at 422 and 544 nm.A K i of 2.4 ��mol/L for competitive cyanide inhibition with respect to guaiacol was observed.
Keywords�� Soybean hull peroxidase   Electronic absorption spectroscopy   Compound      
Received 1999-09-17;
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����.���ǹ�������ø�ĵ������չ������� [J]  �й����ﻯѧ���������ѧ��, 2000,V16(04 ): 499-499
.Electronic Absorption Spectroscopy Properties of Soybean Hull Peroxidase [J]  Chinese Journal of Biochemistry and Molecular Biol, 2000,V16(04 ): 499-499
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http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y2000/V16/I04 /499