首 页期刊介绍编 委 会征稿简则优秀论文期刊订阅广告合作下载中心联系我们留 言 板English
�й����ﻯѧ���������ѧ�� 2000, Vol. 16 Issue (05 ):680-680     DOI:
���� ����Ŀ¼ | ����Ŀ¼ | ����� | �߼����� << [an error occurred while processing this directive] | [an error occurred while processing this directive] >>
������RT-PCR�ⶨ����BMP-2mRNA�Ķ������
������ *
@������$���ҽ�ƴ�ѧ�ڷ����о���!���300070 @����һ$���ҽ�ƴ�ѧ�ڷ����о���!���300070 @����Ұ$���ҽ�ƴ�ѧ�ڷ����о���!���300070 @�ž���$���ҽ�ƴ�ѧ�ڷ����о���!���300070
A Competitive RT-PCR Assay and Quantitation of BMP-2 mRNA
GUO Ruo\|lin,GUO Shan\|yi,BAO Qiu\|ye,ZHANG Jing\|yu ** (Institute of Endocrinology,Tianjin Medical University,Tianjin 300070,China)

ժҪ
�����
�������
Download: PDF (221KB)   HTML 0KB   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ ���������� PCR���� ,��������ת¼ˮƽ������Ķ����о� .�Լ�����­�ǹ�ϸ����RNA�й���̬����� - 2 ( BMP- 2 ,bone morphogenetic protein- 2 ) m RNA����Ϊ�� ,��������BMP- 2����ľ���ģ�� ,��֮Ϊ�ڶ��ս��о����� PCR.PCR��Ӧ����� ,��Ӿ������ ,ɨ�������������ܶ� ,���ع鷽�� .��ݻع鷽�� ,������� 7d Wistar����­���� RNA�� BMP- 2 m RNA����Ϊ 1 .1 2 5amol/��g RNA.�����ʾ ,�ɹ������˴��� BMP- 2����ľ���ģ�� ,�����˿��Բⶨ����­�ǹ�ϸ���� RNA�� BMP- 2 m RNA�����ľ����� PCR���� .
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
������
�ؼ���� ������PCR   ����ģ��   ��ת¼   ����̬�����-2   mRNA     
Abstract�� In order to study the regulation of gene expression and to acquire quantitative information on mRNA of an individual gene,a competitive PCR was established.A competitor of rat BMP 2 (bone morphogenetic protein\|2) gene was constructed.BMP\|2 primer sequences were added to an Eco R��\| Bam H I fragment of V\|erbB oncogene,so the fragment had the same primer sets with BMP 2.Then the competitor and the cDNA that was generated from BMP 2 mRNA by reverse transcription were co\|amplified in one PCR tube,and the products were analyzed by gel electrophoresis.Intensities of the competitor and BMP 2 gene bands were determined by scanning the photo film with a computer scanner.A linear regression equation was established based on data of lg(BMP 2/competitor) and the amounts of the competitor.According to the linear regression equation,the amount of BMP 2 mRNA in total calvarial RNA of normal 7\|days Wistar rats was determined,which was 1.125 amol/��g RNA.The results showed that a competitive PCR had been successfully developed for quantification of the amount of BMP 2 mRNA in total calvarial RNA and satisfied results obtained.
Keywords�� Competitive PCR   Competitor   Reverse transcription   BMP 2   mRNA      
Received 1999-12-23;
���ñ���:   
������.������RT-PCR�ⶨ����BMP-2mRNA�Ķ������ [J]  �й����ﻯѧ���������ѧ��, 2000,V16(05 ): 680-680
.A Competitive RT-PCR Assay and Quantitation of BMP-2 mRNA [J]  Chinese Journal of Biochemistry and Molecular Biol, 2000,V16(05 ): 680-680
���ӱ���:  
http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y2000/V16/I05 /680