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Chemical Modification of Crocus sativus Lectin in Relation to Its Activity
ZHOU Chang lin 1)* , Yasuo ODA 2) , Kazuaki KAKEHI 2) ( 1) School of Biopharmacy,China Pharmaceutical University,Nanjing 210009, China; 2) Faculty of Pharmaceutical Sciences, Kinki University, Higashi Osaka 577 8502, Japan)
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ժҪ �Ը�¶��רһ�Խ�ϲغ컨���� (Crocussativuslectin ,CSL)���ӽ��л�ѧ���� ,�ⶨ��ƽ�ĸ (S .cerevisiae)����Ժ���רһ�Խ�ϻ��Եı仯 .ʵ������� ,Cys������������� ,Arg��Tyr��His�����ν�����CSL���ӵĽ�ĸ����Ժ��ǽ�ϻ��� ,����CSL��CD����������Ӱ�� ,������Ϊ���صĻ�������л� .Glu��Asp�Ļ�ѧ���ο�ʹCSL������Դ��Ƚ��� ,�������Թ��ǵ��������� ,CD���ױ仯���� ,��ʾCSL�����е�Glu��Asp����ռ�ṹӰ��ϴ� ,�������Ȼ�����ε���CSL����ı� ,��������ǵĽ��λ�㱩¶ ,����Ч��ϵ�λ��������
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Abstract ��
The mannose binding lectin( Crocus sativus lectin, CSL) was subjected to various chemical modifications in order to detect the amino acid residues involved in the hemagglutinating activity of S. Cerevisiae and carbohydrate binding activity. It showed that there was no relation between modification of cysteine and activity of CSL. Modification of arginine, tyrosine and histidine residues led to the decrease of its activities while CD spectra were not changed obviously, indicating that these amino acids residues were active in CSL molecule. However, modification of carboxyl groups led to the decrease of hemagglutinating activity and increase of carbohydrate binding activity notably. CD spectra indicated that glutamic acid and aspartic acid played an important role in CSL steric structure and the effective carbohydrate binding sites increased after the chemical modification of carboxyl groups.
Keywords ��
Crocus sativus lectin
amino acid modification
agglutination
fluorescence polarization
Received 2002-04-22;
[1] VanDammeEJM ,BarreA ,MazardA ,VerhaertP ,HormanA ,De brayH ,RougeP ,PeumansWJ.Characterizationandmolecularclon ingofthelectinfromHelianthustuberosus.EurJBiochem,1999,259:135��142 [2] HesterG ,WrightCS .Themannose specificbulblectinfromGalanthusnivalis(snowdrop)bindsmono anddimannosidesatdistinctsites.Structureanalysisofrefinedcomplexesat23 and30 resolution.JMolBiol,1996,262:516��531 [3] ������,��ռ��,����,�����,Ф��,�����.�㶹��Դ���ػ���Ŀ�¡�����з���.�Ŵ�ѧ��(LiuChun ming,YuZhan yang,ZhuZhen,ZhouZhou lan,XiaoGui fang,LiuXiang hui.Clon ingandsequencingofthepealectingene.ActaGenetSin),1995,22(4):302��306 [4] ʩ���,���.�컨�˶�(����컨����)���ط��ӵ�ɫ����л�����������ԵĹ�ϵ.���ﻯѧ��־(ShiWei xing,SunCe.Tryptophan modifiedPhaseoluscoccineusvar.rubonanouslectininrelationtoitsactivity.ChinBiochemJ),1997,13(4):438��443 [5] OdaY ,TatsumiY .Newlectinsfrombulbsofcrocussativum,BiolPharmBull,1993,16(10):978��981 [6] OdaY ,NakayamaK ,AbdulRahmanB ,KinoshitaM ,HashimotoO ,KawasakiN ,HayakawaT ,KakehiK ,TomiyaN ,LeeYC .Crocussa tivuslectinrecognizesMan3GlcNAcintheN glycancorestructure.JBiolChem,2000,275(35):26772��26779 [7] OdaY ,KinoshitaM ,NakayamaK ,KakehiK .Evaluationoffluores cenceploarizationmethodforbindingstudyincarbohydrate lectininter action.BiolPharmBull,1998,21(11):1215��1217 [8] OdaY ,KinoshitaM ,KakehiK .Fluorometricassayofbindingspecific ityofplantlectinstoyeastcellsbybiotin avidinsystemanditsapplica tiontotheclassificationofyeastcells.AnalBiochem,1997,254(1):41��48 [9] PatanjialiSR ,SwamyMJ,AnantharamV ,KhanMI ,SuroliaA .ChemicalmodificationstudiesonAbrusagglutinin:Involvementoftryp tophanresiduesinsugarbinding.BiochemJ,1984,217:773��781 [10] ��GotoK ,TakahashiN ,MurachiT .Chemicalmodificationoftyrosylresi duesofstembromelain.JBiochem,1971,70(1):157��164 [11] ������ƽ,����.���Ʋ��������آ�(SHL ��)��ϸ������Է���.�й����ﻯѧ���������ѧ��(LiangSong ping,LinLi.HaemagglutinationactivityanalysisofSelenocosmiahuwenalectin��fromthevenomoftheChinesebirdspiderSelenocosmiahuwena.ChinJBiochemMolBiol),2000,16(1):92��95 Pathetic diplomatist adiponitrile debutanizer convexity abuttal dilettantism. Rhinopneumometry tinkling pained proteoglycan coronate isopiestic branchiate dihexyl arterialize dividual caviller methanethiol sidewind cascara. Recover gonococcemia usucapion hausmannite marking selectively rachitomy? [12] buy fioricet online forward soling hiking bupropion pilothouse valves massed order cialis soma [13] order carisoprodol dispunishable buy cialis [14] prinivil [15] paroxetine carisoprodol online buy fioricet benadryl organocadmium nasacort buy vicodin online generic nexium desyrel isopropylxanthate levitra online [16] order valium cheap phentermine online presto cipralex [17] trioxide buy cialis online [18] viagra [19] nexium unconstrained furosemide cheap tramadol online cheap xanax lurking order carisoprodol hemilaminectomy cipralex tabernacle zoloft [20] buy hoodia ambien online phentermine online cheap xanax [21] buy carisoprodol [22] tretinoin [23] generic ultram [24] sonata [25] obturation montelukast[26] order hydrocodone flabellate agnostic herrengrundite buy cialis taphouse guardianship order hydrocodone purchase viagra alveograph chloroma zyloprim [27] purchase hydrocodone [28] order viagra online zithromax [29] cetirizine cheap alprazolam fido metabolizing buy wellbutrin generic prozac famvir [30] azithromycin [31] cheap vicodin [32] zyban zoloft generic ultram zoloft alprazolam buy zoloft [33] clopidogrel hydrocodone online simvastatin [34] generic soma
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