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Probable Existence of a Negative Regulation Region Within the 3��UTR of Human Phosphoinositide 3-Kinase�� mRNA
DAI Bing bing, LU Jian, WANG Jia min, MEI Wen han, WANG Chu, QIAN Guan xiang * (Department of Biochemistry, Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China)
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Supporting Info
ժҪ Ϊ̽������֬������ 3 ��ø��(phosphoinositide 3 kinasePI3K��)���� 3��˷Ƿ�������AU�������Ƿ��ڻ���������������� ,����ͨ��������Ϣѧ������������ 3��˷Ƿ����� (UTR)�ڴ���0 9kb��AU������ ,���а��� 4��AU����Ԫ�� ,�Լ� 1�����ڶ����Ƿ�����߶�ͬԴ�ij� 130���������� .��AU��������뱨�����egfp�����ι���pcDNA3 egfp AUR������� .���������ת��NIH 3T3,74 0 2��K5 6 2ϸ�� ,��ʽϸ����egfp�ı����� .PI3K�û��� 3��Ƿ�����AU������������egfp�ı�� 2�� 3�� (P <0 0 1) .���÷��߾���D���RNAת¼�� ,Northernӡ�����������ʾegfp AURmRNA��egfpmRNA���ȶ� .ʵ������ʾ ,PI3K�û��� 3��Ƿ�����AU�������ڿ��ܴ���ת¼��ˮƽ�Ļ����︺������ ,�ø����������һ���̶��ϼ���mRNA��˥��
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Abstract ��
In order to study whether there is a functional regulation region within the 3��untranslational region (UTR) of human phosphoinositide 3 kinase��(PI3K��)mRNA, the 3��UTR was analyzed through bioinformatics. An AU rich region (AUR)of about 0 9 kb was found. Within the AU rich region there are 4 AU rich elements (AREs) and a region which is homologous with many other UTRs. The AU rich region was inserted into the 3��UTR of egfp reporter gene, and pcDNA3 egfp AUR expressing vector was constructed. The expression of egfp containing the 3��UTR of PI3K�� was significantly reduced by 2��3 times in NIH 3T3, 7402 and K562 cells. After treated with actinomycin D (5 ��g/ml) total RNA from stably transfected K562 cells were successively extracted with an interval of 2 hours, and the stability of egfp mRNA was analyzed through Northern blotting. The result showed that the AU rich region of PI3K�� could, to some extent, accelerate the decay of egfp mRNA. These results indicate that there is probably a negative regulation region within the 3��AU rich region of PI3K�� mRNA 3��UTR.
Keywords ��
phosphoinositide 3 kinase�� gene 3��untranslational region
post transcriptional regulation
mRNA stability
Received 2002-11-18;
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�����.PI3K��mRNA 3��Ƿ�������ܴ��ڻ����︺������ [J] �й����ﻯѧ���������ѧ��, 2003,V19(05 ): 630-630
.Probable Existence of a Negative Regulation Region Within the 3��UTR of Human Phosphoinositide 3-Kinase�� mRNA [J] Chinese Journal of Biochemistry and Molecular Biol, 2003,V19(05 ): 630-630
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http://cjbmb.bjmu.edu.cn/CN/ �� http://cjbmb.bjmu.edu.cn/CN/Y2003/V19/I05 /630
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