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�й����ﻯѧ���������ѧ�� 2004, Vol. 20 Issue (06 ):750-750     DOI:
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Construction, Expression and Bioactivity Assay of Recombinant ��-CPP Dimer Gene
JIANG Yu-jun, LI Qing-zhang *, GENG Li-jing, YAN Hong-bo (Life Science & Biotechnique Research Center, Northeast Agricultural University,Harbin 150030,China)

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ժҪ ������Զ��� ���ҵ��������� (�� CPP)�ܹ�����ϸ��Զ������ӵ����ա��ٽ��ܾ���̲���ϸ����ֳ����Ӱ�� .Ϊ�����о�������ѧ���� ,���û��򹤳̷����õ��� CPP�Ķ�������򲢹�����˱������ ,תȾCHOϸ�����ȶ����ϸ��ϵ .����������� ,���������Ծ���ϸ��������Ca2 + ˮƽ��ϸ����ֳ��ϸ������ȷ����Ӱ�� .������ ,���� �� CPP�������ܹ��������Ӿ���ϸ���Ca2 + �����ա��ٽ�Ƣϸ�����ֳ���յ�ijЩ����ϸ��ĵ��� ,���Ź㷺������ѧ���Ժ�DZ�ڵ�ҩ��ѧ����
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Abstract�� Casein phosphopeptide (��-CPP) influences numerous biological processes such as uptake of the bivalent ions and proliferation of the cells. To further investigate biological functions of ��-CPP, eukaryotic expression vector of ��-CPP dimer was constructed and then transfected into CHO cells. After selection, the cell lines expressing ��-CPP dimer were obtained and the recombinant products were identified and purified. Activity assay of the recombinant protein indicated that the recombinant ��-CPP dimer could improve Ca 2+ uptake of sperm,stimulate the proliferation of spleen cells and induce apoptosis of some malignant tumour cells.
Keywords�� ��-casein phosphopeptide   dimer   eukaryotic expression   bioactivity      
Received 2004-01-12;
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��ع��.������ҵ��������Ķ��������Ĺ�������P��������Է��� [J]  �й����ﻯѧ���������ѧ��, 2004,V20(06 ): 750-750
.Construction, Expression and Bioactivity Assay of Recombinant ��-CPP Dimer Gene [J]  Chinese Journal of Biochemistry and Molecular Biol, 2004,V20(06 ): 750-750
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http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y2004/V20/I06 /750