Analysis of cDNAs and Genomic DNA of Snake Venom CTL-like Proteins Revealed An Extraordinary Post-transcriptional Processing Event
ZHA Xiang-dong 1)*, ZHOU Li-zhi 1), HUANG He-sheng 2), LIU Jing 3), XU Kang-sen 4) ( 1) School of Life Sciences, Anhui University, Hefei 230039,China; 2) Department of Pharmacology, Anhui Medical University, Hefei 230022,China; 3) School of Life Sciences, University of Science and Technology of China, Hefei 230026, China; 4) Institute for Control of Pharmaceuticals and Biological Products, Beijing 100050, China)
To better understand the accelerated evolution of snake venom C-type lectin-like proteins (CTL-like proteins) and to investigate the structure-function relationships, PCR was conducted to amplify cDNAs coding for the �� chains of snake venom CTL-like proteins and the genomic DNA of agkisasin ��. The reaction products were cloned and sequenced. The causal relationships between the sequences and the experimental conditions were established. Dot plot analysis and multiple alignments were also performed. The results suggested the existence of a post-transcriptional processing event that was essentially homologous recombination at the RNA level, which might play an important role in the diversity of snake venom CTL-like proteins. This inference would provide a novel perspective for explaining a challenging problem of the post-genomic era: the discrepancy between the limited number of genes and the large collection of cDNAs, which was most prominent with regard to certain snake venom proteins. The genomic DNA of a snake venom C-type lectin-like protein was elucidated and no introns were found in the coding region.
.Analysis of cDNAs and Genomic DNA of Snake Venom CTL-like Proteins Revealed An Extraordinary Post-transcriptional Processing Event [J] Chinese Journal of Biochemistry and Molecular Biol, 2004,V20(06 ): 713-713