����
����Ŀ¼ | ����Ŀ¼ | ����� | ������
<<
[an error occurred while processing this directive]
|
[an error occurred while processing this directive]
>>
�߶�C�������൰��cDNA�������DNA���з�����ʾ�ر��ת¼��ӹ�(Ӣ��)
���� *
@����$���մ�ѧ�����ѧѧԺ!�Ϸ�230039
@����־$���մ�ѧ�����ѧѧԺ!�Ϸ�230039
@�ƺ�ʤ$����ҽ�ƴ�ѧҩ��ϵ!�Ϸ�230022
@����$�й��ѧ������ѧ�����ѧѧԺ!�Ϸ�230026
@�쿵ɭ$�й�ҩƷ������Ʒ�춨��!����100050
Analysis of cDNAs and Genomic DNA of Snake Venom CTL-like Proteins Revealed An Extraordinary Post-transcriptional Processing Event
ZHA Xiang-dong 1)*, ZHOU Li-zhi 1), HUANG He-sheng 2), LIU Jing 3), XU Kang-sen 4) ( 1) School of Life Sciences, Anhui University, Hefei 230039,China; 2) Department of Pharmacology, Anhui Medical University, Hefei 230022,China; 3) School of Life Sciences, University of Science and Technology of China, Hefei 230026, China; 4) Institute for Control of Pharmaceuticals and Biological Products, Beijing 100050, China)
Download: PDF (484KB)
HTML 0KB
Export: BibTeX or EndNote (RIS)
Supporting Info
ժҪ Ϊ�о��߶�C�������൰�Ŀ��ٽ���ƺͽṹ���ܹ�ϵ ,ʹ��PCR�������������ɱ���C�������൰�� ������cDNA�����Լ�agkisasin�µĻ�����DNA ,������Щ����������п�¡�Ͳ��� .�Բ��������������еľ�����������������ϵ���� ,���ҽ��е���ͼ�ȽϺͶ����бȶ� .������ ,���ܴ��ڡ�ת¼��ͬԴ���顱��ת¼����¼� ,���߶�C�������൰�Ķ�������������Ҫ������ .���ڽ��ͻ�����Ŀ�뵰������Ŀ�IJ�����һ�������ʱ�����Ҫ���� ,����һ���IJο���ֵ .�״α����߶�C�������൰�Ļ�����DNA���� ,����δ�������ں���
�ؼ�� ��
C�������൰��
�ں���
ת¼��ͬԴ����
���ٽ�
Abstract ��
To better understand the accelerated evolution of snake venom C-type lectin-like proteins (CTL-like proteins) and to investigate the structure-function relationships, PCR was conducted to amplify cDNAs coding for the �� chains of snake venom CTL-like proteins and the genomic DNA of agkisasin ��. The reaction products were cloned and sequenced. The causal relationships between the sequences and the experimental conditions were established. Dot plot analysis and multiple alignments were also performed. The results suggested the existence of a post-transcriptional processing event that was essentially homologous recombination at the RNA level, which might play an important role in the diversity of snake venom CTL-like proteins. This inference would provide a novel perspective for explaining a challenging problem of the post-genomic era: the discrepancy between the limited number of genes and the large collection of cDNAs, which was most prominent with regard to certain snake venom proteins. The genomic DNA of a snake venom C-type lectin-like protein was elucidated and no introns were found in the coding region.
Keywords ��
C-type lectin-like proteins
intron
post-transcriptional homologous recombination
accelerated evolution
Received 2004-02-26;
����:
����.�߶�C�������൰��cDNA�������DNA���з�����ʾ�ر��ת¼��ӹ�(Ӣ��) [J] �й����ﻯѧ���������ѧ��, 2004,V20(06 ): 713-713
.Analysis of cDNAs and Genomic DNA of Snake Venom CTL-like Proteins Revealed An Extraordinary Post-transcriptional Processing Event [J] Chinese Journal of Biochemistry and Molecular Biol, 2004,V20(06 ): 713-713
���ӱ���:
http://cjbmb.bjmu.edu.cn/CN/ �� http://cjbmb.bjmu.edu.cn/CN/Y2004/V20/I06 /713
[1] DrickamerK .C typelectin likedomains.CurrOpinStructBiol,1999,9:585��590 [2] ChengX ,QianYW ,LiuQD ,LiBXY ,ZhangMW ,LiuJ .Purification,characterization,andcDNAcloningofanewfibrinogenlyticvenomprotein,agkisacutacin,fromAgkistrodonacutusvenom.BiochemBiophysResCommun,1999,265:530��535 [3] MatsuzakiR ,YoshiaraE ,YamadaM ,ShimaK ,AtodaH ,MoritaH .cDNAcloningofIX X BP ,aheterogenoustwo chainanticoagulantproteinfromsnakevenom.BiochemBiophysResCommun,1996,220:382��387 [4] �����,��,�����,����.���ϼ���������11��C���������Ƶ����ǵ�λ��cDNA��¡�����з���.�й����ﻯѧ���������ѧ��(YuHong xiu,XiangKai jun,WangYu jie,LiuJing.CloningandsequencingofelevencDNAsofC typelectin likeproteinsubunitsfromAgkistrodonacutus.ChinJBiochemMolBiol),2003,19(1):64��70 [5] ����,�Ż�Զ,Ф����,����,�쿵ɭ.��������δ֪C�����ص���cDNA��������¡����.���ﻯѧ�����������չ(ZhaXiang dong,ZhangHua yuan,XiaoYa zhong,LiuJing,XuKang sen.Amplifying,cloningandexpressionofthecDNAencodinganunknownC typelectinsuperfamilyproteinfromAgkistrodonacutus.ProgBiochemBiophys),2001,28(5):752��755 [6] CourseauxA ,NahonJL .BirthoftwochimericgenesintheHominidaelineage.Science,2001,291(5507):12931297 [7] ZhaXiang dong,LiuJing,XuKang sheng.cDNAcloning,sequenceanalysisandrecombinantexpressionofakitoninbeta,aC typelectin likeproteinfromAgkistrodonacutus.ActaPharmacolSin,2004,25(3):372��377 [8] MotonoriO ,TakahitoC ,NaokoO ,TomohisaO ,ShosakuH .MolecularevolutionofmyotoxicphospholipasesA2 fromsnakevenom[J].Toxicon.2003,42(8):841-854 [9] ChijiwaT ,HamaiS ,TsubouchiS ,OgawaT ,DeshimaruM ,OdaUedaN ,HattoriS ,KiharaH ,TsunasawaS ,OhnoM .InterislandmutationofanovelphospholipaseA2fromTrimeresurusflavoviridisvenomandevolutionofCrotalinaegroupIIphospholipasesA2.JMolEvol,2003,57(5):546��554 [10] DeshimaruM ,OgawaT ,NagashimaK ,NobuhisaI ,ChijiwaT ,ShimohigashiY ,FukumakiY ,NiwaM ,YamashinaI,HattoriS ,OhnoM .Acceleratedevolutionofcrotalinaesnakevenomglandserineproteases[J].FEBSLett.1996,397:83-88 [11] SiigurE ,AaspolluA ,SiigurJ .SequencediversityofVipealebetinasnakevenomglandserineproteasehomologs resultofalternative splicingorgenomealteration[J].Gene.2001,263:199-203 [12] ��ZhaXiang dong,RenBin,LiuJing,XuKang sen.cDNAcloningandhigh levelexpressionofathrombin likeenzymefromAgkistrodonacutusvenom.MethodsFindingsExpClinPharmacol,2003,25(4):253��257 [13] ��ChungC ,WuBN ,YangCC ,ChangLS .Muscarinictoxin likeproteinsfromTaiwanbandedkrait(Bungarusmulticinctus)venom:purification,characterizationandgeneorganization.BiolChem,2002,383(9):1397��1406 [14] ��LasserreC ,SimonMT ,IshikawaH ,DiriongS ,NguyenVC ,ChristaL ,VernierP ,BrechotC .Structuralorganizationandchromosomallocalizationofahumangene(HIP PAP)encodingaC typelectinoverexpressedinprimarylivercancer.EurJBiochem,1994,224(1):29��38 [15] ��WadaH ,KobayashiM ,SatoR ,SatohN ,MiyasakaH ,ShirayamaY .Dynamicinsertion deletionofintronsindeuterostomeEF 1alphagenes.JMolEvol,2002,54(1):118��128
Vallecula duenna gingivitis delimiting rectalgia quadruplex camel locksmith automaticity repatriate hemihypesthesia; antichain?
Pancreatography cornified rundle.
memorizing orlistat [16] rhamnol order valium online generic xanax kenalog buy carisoprodol lorcet buy alprazolam online portcrayon generic paxil bextra plavix instantaneousness neurontin ultram testosterone sonata [17] generic paxil [18] buy xanax cipralex [19] cheap meridia materialistically famvir purchase soma ludwigite buy diazepam sumatriptan buy carisoprodol retin-a [20] danazol [21] buy levitra online valium generic cialis cheap hydrocodone lansoprazole phlebothrombosis retin-a buy phentermine [22] xenical online singulair [23] celexa cephalexin buy alprazolam imitrex buy fioricet online generic viagra generic zocor neurectopy hydrocodone online buy carisoprodol online phentermine online [24] propecia generic lipitor lightwell generic cialis [25] cetirizine trazodone cheap viagra online [26] prozac online purchase tramadol atorvastatin fluconazole [27]
1������,���ص�,���,�Դ,���,���ӽ�,������,֣����,����.pCAGGs-miR-483��������������miR-483ת����С��ģ�͵Ľ���[J]. �й����ﻯѧ���������ѧ��, 2010,26(05): 478-483
2����ٻ������.��Դ������ɸѡ���ӻ���Inteinϵͳ�Ľ���[J]. �й����ﻯѧ���������ѧ��, 2009,25(03): 282-286
3����;����.PRP8�����ʷ�ʽ����ϵͳ�Ľ���[J]. �й����ﻯѧ���������ѧ��, 2009,25(02): 158-162
4���۰���;����.���ѵ������ں��ӵļ��ӻ��ơ���Դ�ͽ�[J]. �й����ﻯѧ���������ѧ��, 2008,24(06): 512-521
5����������������Ρ�����پ�����������.��Դ�ı���������ԭʼ��ĸ��ϸ�����ڵ���B�����¡������Ŵ�����[J]. �й����ﻯѧ���������ѧ��, 2007,23(10): 849-858
6���ܺ���,�۰���,����.�����ں���(Group �� Intron)�ķֲ��������Ԫ���[J]. �й����ﻯѧ���������ѧ��, 2007,23(08): 605-611
7������ɽ,����,����Ǭ,�ܺ�ΰ,��С��,������.TPO�ں��Ӣ���������ǿ��ѪС������ػ���������ϸ���еı��[J]. �й����ﻯѧ���������ѧ��, 2007,23(07): 537-541
8��������;��۪�;���Ϫ�;�����ª�;����;���»���;����ΰ��;���.��Ѫ����FXIII A�������1�ں�����˳ʽ����Ԫ���ļ�[J]. �й����ﻯѧ���������ѧ��, 2007,23(05): 338-343
9����Т��.����������GIY-YIG��鳲����ø�����ܵ���в��ʱ��ǿת¼ [J]. �й����ﻯѧ���������ѧ��, 2003,19(05 ): 625-625
10������.ת����С������Դ�����ں������е�ȱʧ�����ת¼��Ӱ�� [J]. �й����ﻯѧ���������ѧ��, 1999,15(05 ): 704-704
11���³�.�˰�ϸ����������Ӱ���Grp94�����3��˵ij������� [J]. �й����ﻯѧ���������ѧ��, 1997,13(01 ): 29-29
Copyright 2010 by �й����ﻯѧ���������ѧ��