mPEG Chemical Modification on Erythrocyte Antigens of ABO Blood Group
WANG Jie-xi 1) , TAN Ying-xia 1) , LI Li-li 1) , BAI Yan 2) , ZHANG Yang-pei 1) * ( 1) Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2) Department of Hematology, Chinese PLA 307 Hospital, Beijing 100039, China)
Blood transfusion is a useful clinical treatment ,but blood group incompatibility might cause clinical accident and even death . To discuss the possibility of solving this problem, methoxypolyethylene glycol (mPEG) was used to camouflage erythrocyte antigens. Three mPEG derivatives such as mPEG-BTC, mPEG-ALD and mPEG2-NHS have been tried. The result showed that, comparing with mPEG-ALD and mPEG2-NHS, mPEG-BTC is the most effective material for blood red cell (RBC) camouflage. The covalent modification of RBC with mPEG-BTC effectively obscures antigenic determinants while leaving the RBC structurally and functionally normal and nonimmunogenic. The serological and biochemical characterization of blood group A, B and AB human red blood cell (hRBC) modified with right amount of mPEG-BTC are similar to normal hRBC of group O. Further study indicated that The mPEG modification had no influences on morphology, structures, functions and deformability of hRBC. The results suggest that blood group A,B and AB has been successfully converted to blood group O.The results provide a new method and insight in solving such problems as blood compatibility and rare blood transfusion .