����
����Ŀ¼ | ����Ŀ¼ | ����� | ������
<<
[an error occurred while processing this directive]
|
[an error occurred while processing this directive]
>>
��������Ҷ���(mPEG)�����ڱ���ABOѪ�Ϳ�ԭ
������ *
@������$����ҽѧ��ѧԺҰս��Ѫ�о���!����100850
@̴Ӣϼ$����ҽѧ��ѧԺҰս��Ѫ�о���!����100850
@������$����ҽѧ��ѧԺҰս��Ѫ�о���!����100850
@����$��ž�307ҽԺ��Ѫ��!����100039
@������$����ҽѧ��ѧԺҰս��Ѫ�о���!����100850
mPEG Chemical Modification on Erythrocyte Antigens of ABO Blood Group
WANG Jie-xi 1) , TAN Ying-xia 1) , LI Li-li 1) , BAI Yan 2) , ZHANG Yang-pei 1) * ( 1) Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China; 2) Department of Hematology, Chinese PLA 307 Hospital, Beijing 100039, China)
Download: PDF (206KB)
HTML 0KB
Export: BibTeX or EndNote (RIS)
Supporting Info
ժҪ ��Ѫ��һ�ַdz���Ч���ٴ������ֶ� ,��Ѫ�Ͳ���������Ѫ�����¹� .Ϊ�˽����Ѫ�д��ڵ�Ѫ��ƥ�����ѵ����� ,ʹ�ü�������Ҷ��� (mPEG)��ѧ���η� ,�Ժ�ϸ������Ѫ�Ϳ�ԭ���л�ѧ���� ,�Ӷ�ﵽ�ڱκ�ϸ��Ѫ�Ϳ�ԭ��Ŀ�� .ͨ���mPEG BTC��mPEG ALD��mPEG 2 NHS����mPEG������Ժ�ϸ��A��ԭ��B��ԭ����Ч��ıȽ� ,������mPEG BTC����Ч����� ,������ȫ�ڱκ�ϸ���A��ԭ��B��ԭ ,ʹ���κ��A�͡�B�ͺ�AB�ͺ�ϸ����ֳ���O�ͺ�ϸ����ͬ��Ѫ��Ѫ��ѧ���� ;��һ���о�֤�� ,mPEG BTC���ϸ�����ι� ,�Ժ�ϸ��ṹ�����ܡ���������������ָ��ͳ����ʵȻ�û��Ӱ�� .����ʵ����A��O ,B��O��AB��O��Ѫ���� ,�Ӷ�Ϊ�ٴ���Ѫ����������ƫ�͡�ϡ��Ѫ�͡��������ѵ�����Ľ�� ,�ṩ���µļ���������˼· .
�ؼ�� ��
��ѧ����
��������Ҷ���(mPEG)
��ϸ��Ѫ�Ϳ�ԭ
Abstract ��
Blood transfusion is a useful clinical treatment ,but blood group incompatibility might cause clinical accident and even death . To discuss the possibility of solving this problem, methoxypolyethylene glycol (mPEG) was used to camouflage erythrocyte antigens. Three mPEG derivatives such as mPEG-BTC, mPEG-ALD and mPEG2-NHS have been tried. The result showed that, comparing with mPEG-ALD and mPEG2-NHS, mPEG-BTC is the most effective material for blood red cell (RBC) camouflage. The covalent modification of RBC with mPEG-BTC effectively obscures antigenic determinants while leaving the RBC structurally and functionally normal and nonimmunogenic. The serological and biochemical characterization of blood group A, B and AB human red blood cell (hRBC) modified with right amount of mPEG-BTC are similar to normal hRBC of group O. Further study indicated that The mPEG modification had no influences on morphology, structures, functions and deformability of hRBC. The results suggest that blood group A,B and AB has been successfully converted to blood group O.The results provide a new method and insight in solving such problems as blood compatibility and rare blood transfusion .
Keywords ��
chemical modification
methoxypolyethylene glycol
blood group antigen
Received 2003-10-10;
[1] ScottMD ,MueadKL ,KoumpourasF ,TalbotM ,EatonJW .Chemicalcamouflageofantigenicdeterminants:Stealtherythrocytes.ProcNatlAcadSciUSA ,1997,94:7566��7571 [2] FrickerJ.ConversionofredbloodcellstogroupO [J].Lancet.1996,347:680-681 [3] ����,���,���ز�,������,������.����B��OѪ����IJ�ͬ����������ø�ıȽ�.�й����ﻯѧ���������ѧ��(GaoXin,YangJun,LiSu bo,LiuZe peng,ZhangYang pei.Comparisonofdifferent�� galactosidasesusedintheBtoObloodConversion.ChinJBiochemMolBiol),2003,19(4):504��508 [4] ZhangYang pei,YangJun,GaoXin,JiaYan jun,JiShou ping,GongFeng,LiuZe peng,RenHui ming,LiSu bo,LanJiong cai,CaoQiong.B��Obloodconversion.ChinSciBull,2003,48(1):43��47 [5] BradleyAJ ,TestST ,MuradKL ,MitsuyoshiJ,ScottMD .InteractionsofIgMABOantibodiesandcomplementwithmethoxyPEG modifiedhumanRBCs[J].Transfusion.2001,41:1225-1233 [6] BradleyAJ,MuradKL ,ReganKL ,ReganKL ,ScottMD .Biophysicalconsequencesoflinkerchemistryandpolymersizeonstealtherythrocytes:sizedoesmatter.BiochimBiophysActa,2002,1561:147��158 [7] MuradKL ,MahanyKL ,BrugnaraC ,KuypersFA ,EatonJW ,ScottMD .Structuralandfunctionalconsequencesofantigenicmodulationofredbloodcellswithmethoxypoly(ethyleneglycol).Blood,1999,93(6):2121��2127
Vallecula duenna gingivitis delimiting rectalgia quadruplex camel locksmith automaticity repatriate hemihypesthesia; antichain?
Pancreatography cornified rundle.
memorizing orlistat [8] rhamnol order valium online generic xanax kenalog buy carisoprodol lorcet buy alprazolam online portcrayon generic paxil bextra plavix instantaneousness neurontin ultram testosterone sonata [9] generic paxil [10] buy xanax cipralex [11] cheap meridia materialistically famvir purchase soma ludwigite buy diazepam sumatriptan buy carisoprodol retin-a [12] danazol [13] buy levitra online valium generic cialis cheap hydrocodone lansoprazole phlebothrombosis retin-a buy phentermine [14] xenical online singulair [15] celexa cephalexin buy alprazolam imitrex buy fioricet online generic viagra generic zocor neurectopy hydrocodone online buy carisoprodol online phentermine online [16] propecia generic lipitor lightwell generic cialis [17] cetirizine trazodone cheap viagra online [18] prozac online purchase tramadol atorvastatin fluconazole [19] amoxicillin [20] generic finasteride [21] xanax online bubbly prozac online somatomedin simvastatin cetirizine [22] generic lipitor [23] order valium online buy valium [24] losec finasteride zyrtec hemidiaphoresis gabapentin [25] xenical zyban zyloprim [26] order phentermine clubhand gabapentin cialis online
1���ܳ���.�غ컨���ط��ӻ�ѧ����������ԵĹ�ϵ [J]. �й����ﻯѧ���������ѧ��, 2003,19(01 ): 87-87
2��������.��ˮ������⵰��ø�Ļ�ѧ���� [J]. �й����ﻯѧ���������ѧ��, 2001,17(03 ): 372-372
3�����.ŵ���Ͼ��η��߾��-��¶����ø�Ļ�ѧ���μ��������ĵ��о� [J]. �й����ﻯѧ���������ѧ��, 2000,16(02 ): 227-227
4������.���ǹ�������ø�������ұ����뻯ѧ�����о� [J]. �й����ﻯѧ���������ѧ��, 1999,15(05 ): 802-802
5�����ұ�.��ѧ���ζԷ���Ѻ������ȶ��Լ������в������Ե�Ӱ�� [J]. �й����ﻯѧ���������ѧ��, 1999,15(04 ): 604-604
6��������;ʱ�ɲ�;������;����;�Ƹ���.��ѧ���ο�������ģ��ø��GPX���� [J]. �й����ﻯѧ���������ѧ��, 1999,15(03 ): 0-0
7��������;���ٿ�;�ܺ�;����.�Ҷ����ط���������Բ��ɫ�ԵĹ�ϵ�о� [J]. �й����ﻯѧ���������ѧ��, 1998,14(06 ): 0-0
8���ܺ�;���ٿ�;����;������.Ұ�������صĻ�ѧ������ӫ������о� [J]. �й����ﻯѧ���������ѧ��, 1997,13(05 ): 0-0
9���˼̳�;������;������;�Ƹ���.ճ�����Ͼ���⵰��ø�Ļ�ѧ���� [J]. �й����ﻯѧ���������ѧ��, 1997,13(04 ): 0-0
10��ʩ���;���.�컨�˶�(����컨����)���ط��ӵ�ɫ����л�����������ԵĹ�ϵ [J]. �й����ﻯѧ���������ѧ��, 1997,13(04 ): 0-0
11���ֲ���.�⿵��ľù�������Ͼ�����ˮ��ø�Ļ�ѧ���� [J]. �й����ﻯѧ���������ѧ��, 1997,13(03 ): 302-302
12�����ٿ�;������;�ܺ�;л��ʤ.ˮ���ϻ��ø���Ƽ��ķ�����������Ե���������������� [J]. �й����ﻯѧ���������ѧ��, 1996,12(06 ): 0-0
13����ת��;����.�����˺�ϸ���������绯ø�ȶ��Ե��о� [J]. �й����ﻯѧ���������ѧ��, 1996,12(06 ): 0-0
14���ܺ�;���ٿ�;������.���������ص�ӫ������о� [J]. �й����ﻯѧ���������ѧ��, 1996,12(05 ): 0-0
15������;������;����;�ϻ�;����;��ͬ��;�����.��ѧ���ε���¡����ģ������Ĺ�������ø [J]. �й����ﻯѧ���������ѧ��, 1996,12(05 ): 0-0
Copyright 2010 by �й����ﻯѧ���������ѧ��