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�й����ﻯѧ���������ѧ�� 1992, Vol. 8 Issue (03 ):267-267     DOI:
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Isolation of Human Cardiac Specific Troponin I (CSTn I ) by Affinity Chromatography
Tang,Xin Li,Xiang-chun Yang,Xi-zhen (Jiangsu Institute of Nuclear Medicine,Wuxi 214063)

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ժҪ ��Ca~(2+)������,���Ƶ���C(TnC)�ͼ��Ƶ��ע�(Tn��)���ڸ�Ũ�ȵ�������Һ���γ��ȶ��ĸ�����,��ø��������ܱ�EGTA���롣����TnC��Tn���Ca~(2+)�����Խ�Ϸ�Ӧû���ּ���߼������ͼ�������ԡ����ᴿ���ÿ켡TnC�ͻ����֬�������ż��,�Ʊ�TnC-Sepharose 4B�׺Ͳ�����,���������ļ���ֱ���ᴿ���������Լ��Ƶ��ע�(CSTn��)��SDS-PAGE����������Ϊ29kD���ҡ����䰱�������������˷�����
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Abstract�� In the presence of Ca2+, troponin C(TnC) and troponin I(Tn I ) form a specific binary complex that is stable in high urea concentration and which can be dissociated by ethanedeoxybis- (ethylamine) tetra-acetic acid (EGTA) .Moreover, there is no species or muscle type specificity of Ca2+-dependent interaction between TnC and Tn I .Linking the TnC purified from rabbit fast skeletal muscle with activated agarose gel, TnC-Sepharose 4B affinity chromatographic column was developed for the isolation of CSTn I directly from human heart muscle.The result from SDS-PAGE of isolated CSTn I shows that the molecular weight is about 29kD.The amino acid composition of Tn I was also analysed.
Keywords�� Human cardiac specific troponin I (CSTn I)   Affinity chromatgoraphy   SDS-PAGE   Amino acid composition analysis      
Received 1991-02-02;
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����.�׺Ͳ��������������������Լ��Ƶ��ע�(CSTn��) [J]  �й����ﻯѧ���������ѧ��, 1992,V8(03 ): 267-267
.Isolation of Human Cardiac Specific Troponin I (CSTn I ) by Affinity Chromatography [J]  Chinese Journal of Biochemistry and Molecular Biol, 1992,V8(03 ): 267-267
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http://cjbmb.bjmu.edu.cn/CN/     ��     http://cjbmb.bjmu.edu.cn/CN/Y1992/V8/I03 /267