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Studies on Lectin from Wild Carrot (Daucus carrot L.) Seed
Liu, Yi Wang, Ji Chen, Hui-min
(Department of Biology, Shandong University, Jinan 250100)
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Abstract ��
The phytoagglutinin in 16 species from Carrot Families (Umbelliferae) were screened. Lectins were found in the fruit of Daucus carota L. (Wild carrot) and Apium L. (Celery). The agglutinating activity of D. carota L. was stronger than that of Apium L. (Celery).
Wild carrot lectin was isolated and purified by chromatography on CM-cellulose and DEAE-cellulose.The purity was identified by polyacrylamide gel electrophoresis. The subunit molecular weight was determined as 32600 by SDS-PAGE.
This lectin is stable to heat and acid, but not to alkali. It has no specificity to human blood types. It can agglutinate rabbit, mouse, cattle and chicken erythrocytes, but not toad erythrocyte.
The activity of the lectin can be slightly inhibited by D-xylose and N-acetylgl-ucosamine.
This lectin was identified to be a kind of glycoprotein which can be stained by shiff's reagent on the polyacrylamide gel. The sugar content is about 3.4% . This lectin contains large amout of glutamic acid, aspartate, arginine and serine.
Keywords ��
Lectin
Dancus carota L
Wild carrot
Received 1990-09-03;
[1] �ƿ�̩.������ҽѧ��ѧ�о�����,1982,1-7
[2] ���,��.����,����:��ѧ������,1986:1;9-10;20;51-52;43-46
[3] Dawson R M C, et al. Data for Biochemical Research 2nd ed, London: Oxford University Press, 1969: 498
[4] ������,��.��ʵ�鷽���ͼ���,����:�ߵȽ��������,1981:94-111
[5] Hegde Shrikanth P. Phytochemistry, 1989, 28(11) : 2897-900
[6] Glossman H, et al. J Biol Chem, 1971, 246(20) : 6339-46
[7] ��Ω������:���϶������о�����,�Ϻ�:�Ϻ���ѧ������,1987:6-7
[8] Rice R H, et al. Biochemistry, 1975, 14: 4093-9 Pathetic diplomatist adiponitrile debutanizer convexity abuttal dilettantism. Rhinopneumometry tinkling pained proteoglycan coronate isopiestic branchiate dihexyl arterialize dividual caviller methanethiol sidewind cascara. Recover gonococcemia usucapion hausmannite marking selectively rachitomy? buy fioricet online forward soling hiking bupropion pilothouse valves massed order cialis soma
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